Rodríguez-Solana Raquel, Salgado José Manuel, Domínguez José Manuel, Cortés-Diéguez Sandra
Department of Chemical Engineering, Sciences Faculty, University of Vigo (Campus Ourense), As Lagoas s/n, 32004, Ourense, Spain; Laboratory of Agro-food Biotechnology, CITI-Tecnópole, Parque Tecnológico de Galicia, San Cibrao das Viñas, Ourense, Spain.
Phytochem Anal. 2015 Jan-Feb;26(1):61-71. doi: 10.1002/pca.2537. Epub 2014 Sep 4.
Plants from the Lamiaceae family have been known traditionally for their beneficial health-promoting properties, attributed to their anti-inflammatory, anaesthetic and anti-microbial effects.
The purposes of this study was to characterise the essential oils from four Lamiaceae plants by applying different extraction techniques.
Accelerated solvent (ASE), Soxhlet and supercritical fluid (SFE) extraction methods were compared for their efficiency in obtaining the essential oils from plants. The volatile compounds were identified by GC-MS and the main chemotype was quantified by GC with flame ionisation detection (FID). Phenolic compounds were identified and quantified by HPLC and electrospray ionisation (ESI) with MS/MS.
The essential oils Mentha piperita (ct. menthol/menthone), Rosmarinus officinalis L. (ct. eucalyptol/camphor) and Origanum vulgare (ct. carvacrol/thymol), whereas Thymus vulgaris L. was found to be a pure chemotype (ct. thymol). All three extracts also contained six phenolic compounds. The highest extraction yields were achieved by the Soxhlet and ASE techniques, with M. piperita and R. officinalis L. producing the highest concentrations of rosmarinic and carnosic acids. Finally, it was observed that M. piperita and O. vulgare produced the highest total phenolic content, whereas R. officinalis L. and T. vulgaris L. produced the highest anti-oxidant activity.
The ASE and Soxhlet extraction techniques presented the highest yields of volatile and phenolic compounds, showing their suitability to characterise the chemical profile of aromatic plants.
唇形科植物传统上就因其有益健康的特性而闻名，这些特性归因于其抗炎、麻醉和抗菌作用。
本研究的目的是通过应用不同的提取技术来表征四种唇形科植物的精油。
比较了加速溶剂萃取（ASE）、索氏提取和超临界流体萃取（SFE）方法从植物中获取精油的效率。通过气相色谱 - 质谱联用（GC-MS）鉴定挥发性化合物，并通过带有火焰离子化检测（FID）的气相色谱法定量主要化学型。通过高效液相色谱（HPLC）以及电喷雾电离（ESI）和串联质谱（MS/MS）鉴定和定量酚类化合物。
薄荷（薄荷醇/薄荷酮化学型）、迷迭香（桉叶油素/樟脑化学型）和牛至（香芹酚/百里香酚化学型）的精油，而发现百里香是单一化学型（百里香酚化学型）。所有三种提取物还含有六种酚类化合物。索氏提取和ASE技术获得的提取产率最高，薄荷和迷迭香叶中迷迭香酸和鼠尾草酸的浓度最高。最后，观察到薄荷和牛至的总酚含量最高，而迷迭香叶和百里香叶的抗氧化活性最高。
ASE和索氏提取技术在挥发性和酚类化合物的产率方面表现最高，表明它们适用于表征芳香植物的化学特征。
