Department of thoracic surgery, Cancer Hospital of University of Chinese Academy of Sciences (Zhejiang Cancer Hospital), Hangzhou, Zhejiang, China.
Institute of Cancer and Basic Medicine (ICBM), Chinese Academy of Sciences, Hangzhou, Zhejiang, China.
Asia Pac J Clin Oncol. 2022 Feb;18(1):76-83. doi: 10.1111/ajco.13519. Epub 2021 Mar 25.
To explore the biological function of miR-425/PAK4 axis in proliferation, metastasis, and apoptosis of ovarian cancer (OC) cells.
qRT-PCR and Western blot were adopted to examine miR-425 and PAK4 expressions in OC tissues and cell lines. Cell counting kit-8 (CCK-8) and BrdU assays were applied to detect the proliferation ability of OC cells, and Transwell assay was adopted to assess the migration and invasion of OC cells. Flow cytometry was employed to evaluate the apoptosis of OC cells. The interaction between miR-425 and PAK4 was predicted and verified by bioinformatics analysis and dual luciferase reporter gene assay, respectively.
miR-425 was reduced in OC tissues and cell lines, and its underexpression was in evident correlation with the shorter overall survival time of OC patients. miR-425 impeded OC cell proliferation, migration, and invasion, and accelerated apoptosis. Additionally, PAK4 was validated as the target of miR-425, and the cotransfection of PAK4 reversed the antitumor effect of miR-425.
miR-425 suppresses the proliferation, migration, and invasion of OC cells and enhances apoptosis via inhibiting PAK4, and it is expected to be a prognostic indicator and therapeutic target for the patients with OC.
探讨 miR-425/PAK4 轴在卵巢癌（OC）细胞增殖、转移和凋亡中的生物学功能。
采用 qRT-PCR 和 Western blot 检测 OC 组织和细胞系中 miR-425 和 PAK4 的表达。细胞计数试剂盒-8（CCK-8）和 BrdU 检测法用于检测 OC 细胞的增殖能力，Transwell 检测法用于评估 OC 细胞的迁移和侵袭能力。流式细胞术用于评估 OC 细胞的凋亡。通过生物信息学分析和双荧光素酶报告基因检测分别预测和验证 miR-425 和 PAK4 之间的相互作用。
miR-425 在 OC 组织和细胞系中表达降低，其低表达与 OC 患者的总生存时间较短明显相关。miR-425 抑制 OC 细胞增殖、迁移和侵袭，促进凋亡。此外，PAK4 被验证为 miR-425 的靶基因，PAK4 的共转染逆转了 miR-425 的抗肿瘤作用。
miR-425 通过抑制 PAK4 抑制 OC 细胞的增殖、迁移和侵袭，促进凋亡，有望成为 OC 患者的预后指标和治疗靶点。
