Department of Immunology, Institute for Biological Research "Siniša Stanković" - National Institute of Republic of Serbia, University of Belgrade, Despota Stefana 142, 11000 Belgrade, Serbia.
Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany.
J Neuroimmunol. 2021 May 15;354:577547. doi: 10.1016/j.jneuroim.2021.577547. Epub 2021 Mar 16.
Experimental autoimmune encephalomyelitis (EAE) is classically induced with complete Freund's adjuvant (CFA). The immune response against CFA has a confounding influence on the translational capacity of EAE as a multiple sclerosis model. Here, we compare clinical, cellular and molecular properties between syngeneic spinal cord homogenate (SCH)- and SCH + CFA-immunized Dark Agouti rats. EAE signs were observed earlier and the cumulative clinical score was higher without CFA. Also, a higher number of immune cells infiltrates in the spinal cords was noticed at the peak of EAE without CFA. High spinal cord abundance of CD8CD11bcMHC class II cells was detected in SCH-immunized rats. Myelin basic protein -specific response can be elicited in the cells from the lymph nodes draining the site of SCH immunization. This CFA-free EAE is a reliable multiple sclerosis model.
实验性自身免疫性脑脊髓炎（EAE）通常使用完全弗氏佐剂（CFA）诱导。针对 CFA 的免疫反应对 EAE 作为多发性硬化症模型的转化能力有混杂影响。在这里，我们比较了同种异体脊髓匀浆（SCH）和 SCH+CFA 免疫的 Dark Agouti 大鼠之间的临床、细胞和分子特性。没有 CFA 时，EAE 症状出现更早，累积临床评分更高。此外，在没有 CFA 的 EAE 高峰期，脊髓中浸润的免疫细胞数量更多。在 SCH 免疫大鼠的脊髓中检测到大量 CD8CD11bcMHC Ⅱ类细胞。可以从引流 SCH 免疫部位的淋巴结中的细胞中引发针对髓鞘碱性蛋白的特异性反应。这种无 CFA 的 EAE 是一种可靠的多发性硬化症模型。
