School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, 621010, China.
School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, 621010, China.
J Environ Manage. 2021 Jun 15;288:112247. doi: 10.1016/j.jenvman.2021.112247. Epub 2021 Mar 22.
This study aims to reveal the biodegradation and interaction mechanism of cyclotetramethylenete-tranitramine (HMX) by a newly isolated bacteria. In this study, a bacterial strain (Bacillus aryabhattai) with high efficiency for HMX degradation was used as the test organism to analyze the changes in growth status, cell function, and mineral metabolism following exposure to different stress concentrations (0 and 5 mg L) of HMX. Non-targeted metabonomics was used to reveal the metabolic response of this strain to HMX stress. The results showed that when the HMX concentration was 5 mg L, the removal rate of HMX within 24 h of inoculation with Bacillus aryabhatta was as high as 90.5%, the OD turbidity was 1.024, and the BOD was 225 mg L. Scanning electron microscope (SEM) images showed that the morphology of bacteria was not obvious Variety, Fourier transform infrared spectroscopy (FTIR) showed that the cell surface -OH functional groups drifted, and ICP-MS showed that the cell mineral element metabolism was disturbed. Non-targeted metabonomics showed that HMX induced the differential expression of 254 metabolites (133 upregulated and 221 downregulated). The main differentially expressed metabolites during HMX stress were lipids and lipid-like molecules, and the most significantly affected metabolic pathway was purine metabolism. At the same time, the primary metabolic network of bacteria was disordered. These results confirmed that Bacillus aryabhattai has a high tolerance to HMX and can efficiently degrade HMX. The degradation mechanism involves the extracellular decomposition of HMX and transformation of the degradation products into intracellular purines, amino sugars, and nucleoside sugars that then participate in cell metabolism.
本研究旨在揭示一种新分离的细菌对环四亚甲基四硝胺（HMX）的生物降解和相互作用机制。在本研究中，使用一种对 HMX 具有高效降解能力的细菌菌株（芽孢杆菌）作为测试生物，分析在暴露于不同浓度（0 和 5mg/L）的 HMX 时，生长状态、细胞功能和矿物质代谢的变化。采用非靶向代谢组学方法揭示该菌株对 HMX 胁迫的代谢响应。结果表明，当 HMX 浓度为 5mg/L 时，接种芽孢杆菌 24h 内 HMX 的去除率高达 90.5%，OD 浊度为 1.024，BOD 为 225mg/L。扫描电子显微镜（SEM）图像显示，细菌形态变化不明显，傅里叶变换红外光谱（FTIR）显示细胞表面-OH 功能团漂移，电感耦合等离子体质谱（ICP-MS）显示细胞矿物元素代谢受到干扰。非靶向代谢组学表明，HMX 诱导了 254 种代谢物的差异表达（133 种上调和 221 种下调）。HMX 胁迫下主要差异表达的代谢物为脂类和类脂分子，受影响最显著的代谢途径为嘌呤代谢。同时，细菌的主要代谢网络紊乱。这些结果证实了芽孢杆菌对 HMX 具有较高的耐受性，并能有效地降解 HMX。降解机制涉及 HMX 的细胞外分解和降解产物转化为细胞内嘌呤、氨基糖和核苷糖，然后参与细胞代谢。
