Key Laboratory of Animal Diseases Diagnostic and Immunology, Ministry of Agriculture, MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China.
Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, 225009, China.
Vet Res. 2021 Mar 25;52(1):52. doi: 10.1186/s13567-021-00922-0.
Long non-coding RNAs (lncRNAs) are a new arm of gene regulatory mechanism as discovered by sequencing techniques and follow-up functional studies. The lncRNAs regulation of pseudorabies virus (PRV) infection has rarely been reported so far. Using RNA sequencing analysis, 225 lncRNAs with significant altered expressions in 3D4/21 cells infected with PRV (ZJ01) were identified. Five lncRNAs upregulated in PRV-infected cells were verified in cells infected with different PRV strains by qRT-PCR. By down- and up-regulation of lnc641, the accelerating effect of lnc641 on PRV replication was confirmed. Furthermore, we found that lnc641 regulated PRV replication by inhibiting the JAK-STAT1 pathway. This study suggests that lnc641 could be a new host factor target for developing antiviral therapies against PRV infection.
长链非编码 RNA（lncRNAs）是测序技术和后续功能研究发现的基因调控机制的新分支。迄今为止，关于 lncRNAs 对伪狂犬病病毒（PRV）感染的调节作用鲜有报道。通过 RNA 测序分析，在感染 PRV（ZJ01）的 3D4/21 细胞中鉴定出 225 个表达显著改变的 lncRNAs。通过 qRT-PCR 在感染不同 PRV 株的细胞中验证了上调的 5 个 lncRNAs。通过下调和上调 lnc641，证实了 lnc641 对 PRV 复制的加速作用。此外，我们发现 lnc641 通过抑制 JAK-STAT1 通路来调节 PRV 复制。本研究表明，lnc641 可能成为针对 PRV 感染开发抗病毒治疗的新宿主因子靶标。
