Stord Product Pest Department, Plant Protection Research Institute, Agriculture Research Center, Giza, Egypt.
Zoology Department, Faculty of Science, Al-Azhar University, Cairo, Egypt.
Bull Entomol Res. 2021 Oct;111(5):528-543. doi: 10.1017/S0007485321000225. Epub 2021 Mar 26.
This study was carried out to investigate the efficacy of the non-thermal atmospheric pressure plasma produced with dielectric barrier discharge (APPD) using air as a processing gas and microwave energy to control Tribolium castaneum and Trogoderma granarium adults and larvae in wheat grains. Insects' mortality was found to be power and time-dependent. The results indicated that non-thermal APPD and the microwave have enough insecticidal effect on the target pests. From the bioassay, LT50's and LT90's levels were estimated, T. granarium larvae appeared more tolerant to non-thermal APPD and the microwave energy than adults 7 days post-exposure. The germination percentage of wheat grains increased as the time of exposure to the non-thermal APPD increased. On the contrary, the germination percentage of wheat grains decreased as the time of exposure to the microwave increased. In addition, changes in antioxidant enzyme activities, catalase (CAT), glutathione S-transferase (GST) and peroxidase, in adults and larvae were examined after 24 h post-treatment to non-thermal APPD at 15.9 W power level, which caused 50% mortality. The activity of CAT, GST and lipid peroxide in the treated larvae showed a significant increase post-exposure to the non-thermal APPD at 15.9 W power level. On the other hand, no significant change in GSH-Px activity was observed. Reductions in the level of glutathione (GSH) and protein content occurred in treated larvae in comparison with the control.
本研究旨在探讨使用空气作为处理气体并利用微波能量产生的非热常压等离子体（APPD）对小麦中仓储物害虫赤拟谷盗和玉米象成虫和幼虫的控制效果。发现昆虫死亡率与功率和时间有关。结果表明，非热 APPD 和微波对目标害虫具有足够的杀虫效果。通过生物测定，估计了 LT50 和 LT90 的水平，T. granarium 幼虫在暴露于非热 APPD 和微波能量 7 天后比成虫更能耐受。暴露于非热 APPD 的时间越长，小麦籽粒的发芽率越高。相反，暴露于微波的时间越长，小麦籽粒的发芽率越低。此外，在以 15.9 W 功率水平处理非热 APPD 24 小时后，检查了成虫和幼虫中抗氧化酶活性（CAT、GST 和过氧化物酶）的变化，导致 50%的死亡率。暴露于 15.9 W 功率水平的非热 APPD 后，处理幼虫的 CAT、GST 和脂质过氧化物的活性显著增加。另一方面，GSH-Px 活性没有明显变化。与对照相比，处理幼虫的谷胱甘肽（GSH）和蛋白质含量水平降低。
