Department of Chemistry and Center of Excellence for Innovation in Chemistry (PERCH-CIC), Faculty of Science, Mahidol University, Rama VI Road, Bangkok 10400, Thailand.
Centre for Advanced Research in Sciences(CARS), University of Dhaka, Dhaka 1000, Bangladesh.
Fitoterapia. 2021 Jun;151:104885. doi: 10.1016/j.fitote.2021.104885. Epub 2021 Mar 22.
Four previously undescribed tetrahydrofuran lignans, named anorisols A-D (1-4) and fourteen known compounds (5-18) were isolated from the roots, stems, leaves and twigs of Anogeissus rivularis. The chemical structures were elucidated on the basis of their spectroscopic data and by comparison with the literature data. The absolute configurations of 1-4 were established by comparison of the experimental ECD spectra with the calculated ECD spectra. Some isolated compounds were evaluated for their cytotoxic activity as well as anti-HIV-1 activity employing reverse transcriptase (RT) and syncytium reduction assays using the MC99 virus in 1A2 cell line systems. Compound 6 displayed the most potent activity in syncytium inhibition assay with effective concentration at 50% (EC) value of 13.3 μM (SI >3.0). In the reverse transcriptase assay, compound 1 exhibited moderate activity with IC value of 213.9 μM.
从 Anogeissus rivularis 的根、茎、叶和小枝中分离得到了四个以前未描述的四氢呋喃木脂素，分别命名为 anorisols A-D（1-4）和 14 个已知化合物（5-18）。根据其光谱数据和与文献数据的比较，阐明了化学结构。通过比较实验 ECD 光谱与计算 ECD 光谱，确定了 1-4 的绝对构型。一些分离得到的化合物在逆转录酶（RT）和使用 MC99 病毒在 1A2 细胞系系统中的合胞体还原测定中进行了细胞毒性和抗 HIV-1 活性评估。化合物 6 在合胞体抑制测定中显示出最强的活性，有效浓度在 50%（EC）值为 13.3μM（SI>3.0）。在逆转录酶测定中，化合物 1 表现出中等活性，IC 值为 213.9μM。
