Wijaya Amanda Natalie, Margiana Ria, Kusumaningtyas Sasanthy, Furqonita Deswaty
Undergraduate Program of Medical Education, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia.
Department of Anatomy, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia.
Anat Cell Biol. 2021 Jun 30;54(2):225-231. doi: 10.5115/acb.20.314.
Learning anatomy in medical school is still closely based on the use of cadavers. The burial of preserved cadaver poses a problem, specifically, it contaminates the soil with formalin. Many studies have been conducted to find an alternative fixative to update or modify formalin usage. One of them is ethanol-glycerin (EG), which suggests promising results. Despite that fact, there has yet to be any research comparing the decomposition rate between EG and formalin. This study is conducted to compare the rate of decomposition between the two fixative solutions, EG and 4% formalin on the hind limb of mice. The mice were first preserved using a standard primary fixative solution which is 10% formalin, following that procedure is preservation using advanced fixative solution, EG or 4% formalin. Upon completing the preservation steps, the mice were buried for 6 weeks and observed weekly. The stages of decomposition were assessed semi-quantitatively depending on its appearance. The hind limbs of mice that were fixed with EG solution managed to reach the last stage of decomposition, dry & remains, while the 4% formalin group of mice still remained in the previous stage, advanced decay. It is concluded that the mice hind limbs that have been previously preserved with EG advanced fixative solution has a faster decomposition rate compared to 4% formalin.
在医学院学习解剖学仍然紧密依赖于尸体的使用。保存的尸体埋葬带来了一个问题，具体而言，它会用福尔马林污染土壤。已经进行了许多研究以寻找替代固定剂来更新或改变福尔马林的使用。其中之一是乙醇 - 甘油（EG），其显示出有前景的结果。尽管如此，尚未有任何研究比较EG和福尔马林之间的分解速率。本研究旨在比较两种固定剂溶液EG和4％福尔马林对小鼠后肢的分解速率。首先使用标准的初级固定剂溶液10％福尔马林保存小鼠，随后使用高级固定剂溶液EG或4％福尔马林进行保存。完成保存步骤后，将小鼠埋葬6周并每周观察。根据其外观半定量评估分解阶段。用EG溶液固定的小鼠后肢成功达到分解的最后阶段，干燥及残骸，而4％福尔马林组的小鼠仍处于前一阶段，高度腐烂。得出的结论是，与4％福尔马林相比，先前用EG高级固定剂溶液保存的小鼠后肢具有更快的分解速率。
