Kvinnsland S
Institute of Anatomy University of Bergen, Norway.
Acta Anat (Basel). 1988;131(3):231-4. doi: 10.1159/000146521.
To evaluate the capacity for growth of cartilage in vitro, the cartilaginous nasal septum was cultured in a serum-free medium in short- and long-term incubation periods. The proliferative activity was assessed by relating counts per minute (cpm) obtained in the liquid scintillater after [3H]-thymidine incorporation with the DNA content of the tissue under investigation. Cell proliferation was interpreted as being synonymous with the DNA synthesizing activity or the cpm/microgram DNA ratio. Short-term incubation (24 h) tended to show the same proliferative activity which we have previously found in vivo and as autotransplants, showing that the presence of serum seems to be of little importance under these conditions. Long-term incubation in a serum-free medium tended to show a gradual loss of proliferative activity, emphasizing the need for either serum or other growth-promoting factors as necessary for continued normal proliferation of cartilage in vitro.
为评估软骨在体外的生长能力，将鼻中隔软骨在无血清培养基中进行短期和长期培养。通过将掺入[³H] - 胸腺嘧啶核苷后在液体闪烁计数器中获得的每分钟计数（cpm）与所研究组织的DNA含量相关联来评估增殖活性。细胞增殖被解释为与DNA合成活性或cpm/微克DNA比值同义。短期培养（24小时）倾向于显示出我们之前在体内和自体移植中发现的相同增殖活性，这表明在这些条件下血清的存在似乎不太重要。在无血清培养基中进行长期培养倾向于显示增殖活性逐渐丧失，强调了血清或其他生长促进因子对于软骨在体外持续正常增殖的必要性。
