Sun Bo, Jiang Jie, Zhu Xinyan, Yang Dan, Cui Zhenyu, Zhang Yu, Zhang Minbo, Qian Yiting, Liu Ruilin, Yang Wenzhuo
Department of Gastroenterology and Hepatology, Institute of Digestive Disease, Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, P.R. China.
Department of Gastroenterology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, P.R. China.
Exp Ther Med. 2021 May;21(5):471. doi: 10.3892/etm.2021.9902. Epub 2021 Mar 8.
Hepatic ischemia-reperfusion injury (HIRI) often occurs following surgical procedures such as liver resection and transplantation. However, despite its clinical prominence, to the best of our knowledge, there remain no effective strategies to treat HIRI. Therefore, the aim of present study was to identify therapeutic agents that can exert beneficial effects against HIRI. The present study found that following hepatic IR modeling in mice, gastrodin (Gas) pretreatment improved the IR outcomes in terms of the serum biochemical indexes (alanine transaminase and aspartate transaminase), tissue biochemical indexes (superoxide dismutase, malondialdehyde and reduced glutathione content) and tissue pathology (H&E staining). In addition, compared with those in the IR + vehicle group, the IR + Gas group showed upregulated expression levels of nuclear erythroid 2-related factor 2, heme oxygenase 1 and Bcl-2 as detected by western blotting and reverse transcription-quantitative PCR. The mRNA and protein expression levels of Bax and caspase-3 were downregulated in the IR + Gas group compared with the IR + vehicle group. Concurrently, no significant differences were observed in the parameters between the Sham + vehicle and the Sham + Gas groups, indicating that Gas pretreatment may not cause liver damage. In conclusion, the findings of the present study revealed that Gas pretreatment exerted a protective effect in HIRI through both antioxidant and anti-apoptotic mechanisms.
肝缺血再灌注损伤（HIRI）常发生于肝切除和肝移植等外科手术后。然而，尽管其在临床上较为突出，但据我们所知，目前仍没有有效的治疗HIRI的策略。因此，本研究的目的是确定能够对HIRI产生有益作用的治疗药物。本研究发现，在小鼠肝脏缺血再灌注模型建立后，天麻素（Gas）预处理在血清生化指标（丙氨酸转氨酶和天冬氨酸转氨酶）、组织生化指标（超氧化物歧化酶、丙二醛和还原型谷胱甘肽含量）以及组织病理学（苏木精-伊红染色）方面改善了缺血再灌注结果。此外，与缺血再灌注+赋形剂组相比，通过蛋白质免疫印迹法和逆转录-定量聚合酶链反应检测发现，缺血再灌注+Gas组核因子E2相关因子2、血红素加氧酶1和Bcl-2的表达水平上调。与缺血再灌注+赋形剂组相比，缺血再灌注+Gas组中Bax和半胱天冬酶-3的mRNA和蛋白质表达水平下调。同时，假手术+赋形剂组和假手术+Gas组之间的参数未观察到显著差异，表明Gas预处理可能不会导致肝损伤。总之，本研究结果表明，Gas预处理通过抗氧化和抗凋亡机制对HIRI发挥了保护作用。
