单克隆抗体检测膀胱癌组织中的汤姆森-弗里德赖希抗原。

Thomsen-Friedenreich antigen in bladder cancer tissues detected by monoclonal antibody.

作者信息

Yokoyama M, Ohoka H, Oda H, Oda T, Utsumi S, Takeuchi M

机构信息

Department of Urology, Ehime University School of Medicine.

出版信息

Hinyokika Kiyo. 1988 Feb;34(2):255-8.

PMID:3376817

Abstract

Monoclonal antibodies against Thomsen-Friedenreich antigen (T-Ag) were obtained by the hybridoma technique. The expression of T-Ag in 73 specimens of bladder cancer was examined by the immunofluorescence method using these monoclonal antibodies. Seventeen (53%) of grade I, 18 (44%) of grade II and 5 (50%) of grade III were diffusely stained with anti-T. Of the T-negative tumors, 14 (44%) of grade I and 13 (32%) of grade II showed positive staining after neuraminidase treatment (cryptic T-positive) while only 1 (10%) of grade III was cryptic T-positive. On the contrary, only 1 case (3%) of grade I was cryptic T-negative while 10 (24%) of grade II and 4 (40%) of grade III (were. The T-antigen expression and the histological grades correlated significantly (p less than 0.05). Twenty-eight (70%) of the T-positive cases and 9 (60%) of the cryptic T-negative cases recurred while only 3 (11%) of the cryptic T-positive cases did (p less than 0.01). The monoclonal antibodies were more useful than peanut lectin (PNA) or conventional polyclonal antibodies on the detection of T-antigen.

摘要

通过杂交瘤技术获得了抗汤姆森 - 弗里德赖希抗原（T抗原）的单克隆抗体。使用这些单克隆抗体，通过免疫荧光法检测了73例膀胱癌标本中T抗原的表达情况。I级肿瘤中有17例（53%）、II级中有18例（44%）、III级中有5例（50%）被抗T抗体弥漫性染色。在T抗原阴性的肿瘤中，I级的14例（44%）和II级的13例（32%）在神经氨酸酶处理后显示阳性染色（隐匿性T抗原阳性），而III级中只有1例（10%）为隐匿性T抗原阳性。相反，I级中只有1例（3%）为隐匿性T抗原阴性，II级中有10例（24%），III级中有4例（40%）。T抗原表达与组织学分级显著相关（p小于0.05）。T抗原阳性病例中有28例（70%）复发，隐匿性T抗原阴性病例中有9例（60%）复发，而隐匿性T抗原阳性病例中只有3例（11%）复发（p小于0.01）。在检测T抗原方面，单克隆抗体比花生凝集素（PNA）或传统多克隆抗体更有用。