Ohoka H, Shinomiya H, Yokoyama M, Ochi K, Takeuchi M, Utsumi S
Urol Res. 1985;13(2):47-50. doi: 10.1007/BF00261564.
TUR specimens of non-invasive transitional-cell carcinomas were examined for their expression of Thomsen-Friedenreich (T) antigen by indirect immunofluorescence staining using rabbit IgG antibody which was raised with desialated glycophorin. Nine (45%) out of 20 tumors of low grade (Grade I and II), and 5 (56%) of 9 tumors of high grade (III) were diffusely stained with anti-T (T-positive), whereas T-antigen in normal tissue was cryptic and stained only after neuraminidase treatment (cryptic T-positive). Of the T-negative tumors, 9 (45%) of the low grade but only one of the high grade tumors, were stained positively for the cryptic T-antigen. The rest of the tumors were devoid of the cryptic T-antigen. Eighty percent of both Tumors expressing T-antigen and those lacking the cryptic T-antigen recurred within two years. Recurrence was not influenced by initial histological grade.
通过使用由去唾液酸化血型糖蛋白免疫家兔产生的IgG抗体进行间接免疫荧光染色，检测非侵袭性移行细胞癌经尿道切除术（TUR）标本中汤姆森-弗里德赖希（T）抗原的表达。20例低级别（I级和II级）肿瘤中有9例（45%），9例高级别（III级）肿瘤中有5例（56%）被抗-T抗体弥漫性染色（T阳性），而正常组织中的T抗原是隐蔽的，仅在神经氨酸酶处理后才被染色（隐蔽性T阳性）。在T阴性肿瘤中，低级别肿瘤有9例（45%），但高级别肿瘤只有1例对隐蔽性T抗原呈阳性染色。其余肿瘤缺乏隐蔽性T抗原。表达T抗原和缺乏隐蔽性T抗原的肿瘤在两年内复发率均为80%。复发不受初始组织学分级的影响。
