Igawa M
Hinyokika Kiyo. 1986 Nov;32(11):1663-76.
The significance of Thomsen-Friedenreich antigen (T-Ag) in bladder carcinomas and multiple mucosal biopsies was studied. The T-antigen, a precursor of MN blood group antigen, is not found in normal cells, in which T-antigen is cryptic (cryptic T-Ag) but can be unmasked with neuraminidase digestion. Specimens of 27 main tumors and 201 mucosal biopsies from 28 patients with carcinoma in situ or microinvasion of carcinoma in situ were examined for the expression of T-antigen by Avidin-Biotin-Peroxidase Complex (ABC) method with peanut agglutinin (PNA). The T-Ag-positive rate was 22% for G2 tumors, 56% for G3 tumors, while cryptic T-Ag-positive rate was 86% for G2 tumors and 50% for G3 tumors. The correlation between T-antigen, cryptic T-antigen and histologic grade was not statistically significant. The T-Ag-positive rate in mucosal biopsies was 43% in microinvasion of carcinoma in situ, 11% in carcinoma in situ, 17% in transitional cell carcinoma, 8% in dysplasia, 33% in squamous metaplasia, 33% in proliferative cystitis and 13% in normal epithelium. Of histological findings, microinvasion of carcinoma in situ showed a significantly higher T-Ag-positive rate than carcinoma in situ and normal epithelium (P less than 0.005, P less than 0.001). The cryptic T-Ag-positive rate in mucosal biopsies was 38% in microinvasion of carcinoma in situ, 74% in carcinoma in situ, 100% in transitional cell carcinoma, 82% in dysplasia, 100% in squamous metaplasia, 100% in proliferative cystitis and 96% in normal epithelium. Of the histological findings, carcinoma in situ, proliferactive cystitis and normal epithelium showed a significantly higher cryptic T-Ag-positive rate than microinvasion of carcinoma in situ (P less than 0.025, P less than 0.05, P less than 0.001). Microinvasion of carcinoma in situ expressed the T-Ag (43%), the cryptic T-Ag (21%) and lacked the cryptic T-Ag (36%). Microinvasion of carcinoma in situ showed statistically significant difference in the mode of T-Ag and cryptic T-Ag expression than other histological types in mucosal biopsies, including carcinoma in situ (P less than 0.001).(ABSTRACT TRUNCATED AT 400 WORDS)
研究了汤姆森 - 弗里德赖希抗原（T抗原）在膀胱癌及多处黏膜活检中的意义。T抗原是MN血型抗原的前体，在正常细胞中不存在，正常细胞中的T抗原是隐蔽的（隐蔽性T抗原），但可通过神经氨酸酶消化使其暴露。采用抗生物素蛋白 - 生物素 - 过氧化物酶复合物（ABC）法及花生凝集素（PNA）检测了28例原位癌或原位癌微浸润患者的27个主要肿瘤标本和201个黏膜活检标本中T抗原的表达。G2级肿瘤的T抗原阳性率为22%，G3级肿瘤为56%，而G2级肿瘤的隐蔽性T抗原阳性率为86%，G3级肿瘤为50%。T抗原、隐蔽性T抗原与组织学分级之间的相关性无统计学意义。黏膜活检中，原位癌微浸润的T抗原阳性率为43%，原位癌为11%，移行细胞癌为17%，发育异常为8%，鳞状化生为33%，增殖性膀胱炎为33%，正常上皮为13%。在组织学检查结果中，原位癌微浸润的T抗原阳性率显著高于原位癌和正常上皮（P<0.005，P<0.001）。黏膜活检中，原位癌微浸润的隐蔽性T抗原阳性率为38%，原位癌为74%，移行细胞癌为100%，发育异常为82%，鳞状化生为100%，增殖性膀胱炎为100%，正常上皮为96%。在组织学检查结果中，原位癌、增殖性膀胱炎和正常上皮的隐蔽性T抗原阳性率显著高于原位癌微浸润（P<0.025，P<0.05，P<0.001）。原位癌微浸润表达T抗原（43%）、隐蔽性T抗原（21%）且缺乏隐蔽性T抗原（36%）。原位癌微浸润在黏膜活检中的T抗原和隐蔽性T抗原表达模式与其他组织学类型（包括原位癌）相比有统计学显著差异（P<0.001）。（摘要截于400字）
