López Pacheco Manuel Abraham, Báez Rojas José Javier, Castro-Ramos Jorge, Villa Manríquez José Fabian, Esmonde-White Karen
Instituto Nacional de Astrofísica Óptica y Electrónica (INAOE), Tonanzintla, Mexico.
Kaiser Optical System, Inc., Ann Arbor, MI, USA.
Heliyon. 2021 Mar 17;7(3):e05797. doi: 10.1016/j.heliyon.2020.e05797. eCollection 2021 Mar.
Capsaicin is an active compound in hot peppers. It has been studied for its health benefits for humans. Optical spectroscopy is an important tool for determining the optical properties or chemical composition of matter. The aim of this research is proposing an optical method to identify and quantify capsaicin in the visible range. To achieve this goal, we combined absorption and diffuse reflectance spectroscopy techniques to compute the extinction coefficient. Moreover, the concentration of the analytes was determined using the optical properties of capsaicin. Our method is a promising tool for developing a classification of capsaicin according to its percentage in chilies. The extinction coefficients are reported for 507nm and 663nm, which are the most significative. In addition, the coefficients to build the mathematical model for capsaicin are reported for Kubelka-Munk model. Finally, a comparison between capsaicin vs chilies spectra was obtained to identify spectral response. Diffuse reflectance signals allowed the identification of capsaicin and opened the possibility of this fast and easy to do method for classification and quantification of bioactive compounds.
辣椒素是辣椒中的一种活性化合物。人们对其对人类健康的益处进行了研究。光谱学是确定物质光学性质或化学成分的重要工具。本研究的目的是提出一种在可见光范围内识别和量化辣椒素的光学方法。为实现这一目标，我们结合吸收光谱和漫反射光谱技术来计算消光系数。此外，利用辣椒素的光学性质测定分析物的浓度。我们的方法是根据辣椒素在辣椒中的百分比对其进行分类的一种很有前景的工具。报告了507nm和663nm处的消光系数，这两个波长最为显著。此外，还报告了用于库贝尔卡-蒙克模型的构建辣椒素数学模型的系数。最后，对辣椒素与辣椒光谱进行了比较以识别光谱响应。漫反射信号有助于识别辣椒素，并为这种快速且易于操作的生物活性化合物分类和量化方法提供了可能性。
