Faculty of Physics, Semnan University, Semnan, 35195-363, Iran.
Department of Medical Biotechnology and Nanotechnology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Sci Rep. 2024 Nov 30;14(1):29812. doi: 10.1038/s41598-024-81692-5.
Green synthesis using plant extracts such as Ocimum basilicum L. (OBL) seed has attracted attention due to its sustainable and environmentally friendly nature. In this study, ZnO-MgO-MnO nanocomposites were synthesized using OBL seed extract at two different calcination temperatures of 500 °C and 600 °C, and evaluated in terms of photocatalytic application and cytotoxicity. Phytochemicals in OBL seeds act as reducing and masking agents in the production route, which leads to the formation of nanomaterials with unique properties. Characterization techniques, including XRD, FE-SEM, and DRS were used to analyze the structural, morphological, and optical features of the nanocomposite. XRD results confirmed increasing crystal size from ~ 32 nm (500 °C) to ~ 84 nm (600 °C). Also, FE-SEM images showed formation of irregularly shaped nanocomposites and the EDX spectra of the samples confirmed the presence of zinc, magnesium, manganese, and oxygen elements. The photocatalytic behavior of the nanocomposite cacined at 500 °C was investigated for different organic pollutants. Removal percentages of 97% for Eriochrome Black T dye (pH = 10 for 90 min), 99% for methylene blue dye (pH = 10 for 60 min), 89% for methyl orange dye (pH = 10 for 105 min), and 86.9% for Rhodamine B dye (pH = 3 for 90 min) were obtained. Moreover, the cytotoxicity of the synthesized nanocomposite at 500 °C was evaluated on the 4T1 cell line to investgate its effect on biological systems, and the IC value was obtained around 323 µg/mL.
采用植物提取物（如罗勒种子）的绿色合成因其可持续和环保的特性而受到关注。在这项研究中，使用罗勒种子提取物在 500°C 和 600°C 两种不同的煅烧温度下合成了 ZnO-MgO-MnO 纳米复合材料，并从光催化应用和细胞毒性方面进行了评估。罗勒种子中的植物化学物质在生产过程中充当还原剂和掩蔽剂，导致形成具有独特性质的纳米材料。采用 XRD、FE-SEM 和 DRS 等技术对纳米复合材料的结构、形态和光学特性进行了分析。XRD 结果证实，晶体尺寸从32nm（500°C）增加到84nm（600°C）。此外，FE-SEM 图像显示出不规则形状的纳米复合材料的形成，并且样品的 EDX 光谱证实了锌、镁、锰和氧元素的存在。在 500°C 下煅烧的纳米复合材料的光催化行为研究了不同的有机污染物。Eriochrome Black T 染料（pH=10 时 90 分钟）的去除率为 97%，亚甲蓝染料（pH=10 时 60 分钟）的去除率为 99%，甲基橙染料（pH=10 时 105 分钟）的去除率为 89%，罗丹明 B 染料（pH=3 时 90 分钟）的去除率为 86.9%。此外，在 4T1 细胞系上评估了在 500°C 下合成的纳米复合材料的细胞毒性，以研究其对生物系统的影响，并且获得了大约 323µg/mL 的 IC 值。
