Hsueh An-Ju, Park Sunho, Satoh Tatsuya, Shimizu Tetsuro, Koiwai Kazuki, Nakashima Masahiro, Morimoto Yuji, Kinoshita Manabu, Suzuki Hiroaki
Graduate School of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8573, Japan.
Department of Immunology and Microbiology, National Defense Medical College, Namiki 3-2, Tokorozawa 359-8513, Japan.
Anal Chem. 2021 Apr 6;93(13):5577-5585. doi: 10.1021/acs.analchem.1c00227. Epub 2021 Mar 26.
A microdevice for the measurement of the respiratory activity of cells was fabricated using a microfabricated Clark-type oxygen electrode. The oxygen electrode was completed in a dry state and was activated by introducing water necessary for the reduction of oxygen in the form of water vapor through an oxygen-permeable membrane, which significantly facilitated handling of the device even by nonspecialists. The use of a thin paper layer stabilized the current response and enabled stable continuous operation of the oxygen electrode without current disturbance caused by the evaporation of water. The microdevice was tested in some model experiments including the measurement of the respiratory activity of (), evaluation of the efficacy of antibiotics, and measurement of the antibacterial activity of neutrophils, all of which demonstrated that the consumption of dissolved oxygen by cells can be monitored clearly by following an easy procedure for the preparation of the measurements.
一种用于测量细胞呼吸活性的微型装置是使用微制造的克拉克型氧电极制造的。氧电极在干燥状态下完成，并通过透氧膜以水蒸气的形式引入还原氧所需的水来激活，这极大地便于非专业人员操作该装置。使用薄纸层稳定了电流响应，并使氧电极能够稳定连续运行，而不会因水蒸发引起电流干扰。该微型装置在一些模型实验中进行了测试，包括测量（）的呼吸活性、评估抗生素的疗效以及测量中性粒细胞的抗菌活性，所有这些都表明，通过遵循简单的测量准备程序，可以清楚地监测细胞对溶解氧的消耗。
