Wise R R, Naylor A W
Anal Biochem. 1985 Apr;146(1):260-4. doi: 10.1016/0003-2697(85)90424-5.
A calibration procedure for a Clark-type oxygen electrode over a wide range of temperatures is described. The autoxidation of duroquinol (2,3,5,6-tetramethyl-1,4-benzenediol) was used to verify the electrode's ability to accurately sense the total amount of dissolved O2 in an aqueous buffer. Electrode response time was measured by using oxygenated ethanol to deliver a rapid increase in O2 concentration to the reaction medium. An oxygen-producing system (spinach thylakoids) was utilized to test the range of O2-evolution rates able to be sensed. It was concluded that a Clark-type oxygen electrode has the absolute sensitivity, rapidity, and range necessary to accurately track rates of O2 production or consumption from 5 to 45 degrees C.
本文描述了一种用于克拉克型氧电极在较宽温度范围内的校准程序。使用杜罗醌（2,3,5,6-四甲基-1,4-苯二酚）的自氧化来验证电极准确检测水性缓冲液中溶解氧总量的能力。通过使用含氧乙醇向反应介质快速增加氧气浓度来测量电极响应时间。利用一个产氧系统（菠菜类囊体）来测试能够检测到的氧气释放速率范围。得出的结论是，克拉克型氧电极具有绝对灵敏度、快速性和范围，能够在5至45摄氏度准确跟踪氧气产生或消耗的速率。
