Embrapa Caprinos e Ovinos, Sobral, Brazil.
Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, Jaboticabal, Brazil.
Biopreserv Biobank. 2022 Dec;20(6):493-501. doi: 10.1089/bio.2021.0066. Epub 2021 Nov 5.
This study assessed the outcomes of nonsurgical embryo recovery (NSER) after superovulation (SOV) in five locally adapted Brazilian breeds of sheep and goats. The objective was to evaluate the feasibility and efficiency of using SOV combined with a less-invasive embryo collection technique for supplying the Brazilian animal gene bank with germplasm from specific genotypes of interest. Morada Nova ( = 20), Santa Inês ( = 20), and Somalis ( = 20) ewes received an intravaginal progesterone (330 mg) device for 9 days, while Canindé ( = 15) and Moxotó ( = 15) goats received an intravaginal medroxyprogesterone acetate (60 mg) device for 6 days. All females received 133 mg of porcine follicle-stimulating hormone (pFSH) administrated in six decreasing doses 12 hours apart, starting 60 hours before device removal, plus 37.5 μg of d-cloprostenol at the fifth and sixth pFSH dose. Donors in estrus were mated with fertile males. The corpora lutea (CL) number was assessed by ultrasonography 1 day before NSER. On day 6.5 or 7 after estrus, NSER was performed following hormonally induced cervical relaxation. A total of 97% of sheep and 90% of goats responded with estrus, and among those, 91% of sheep and 85% of goats presented a CL. In ewes, the numbers of CL were greater ( < 0.05) in the Santa Inês breed, while similar ( > 0.05) CL numbers were found among the goat breeds. All viable embryos were freezable (excellent and good quality) and the number per donor was 7.8 for sheep and 4.9 for goats. All parameters of NSER efficiency, embryo yield, and fertility post-NSER did not differ ( > 0.05) between breeds among each species. The SOV-NSER procedures applied for an embryo biobank supply of locally adapted Brazilian breeds of small ruminants were efficient regarding production of cryopreservable embryos, and preservation of donor fertility. Therefore, SOV followed by NSER is recommended for embryo biobank assembly in sheep and goats.
本研究评估了超数排卵（SOV）后非手术胚胎回收（NSER）在 5 个巴西本地绵羊和山羊品种中的结果。目的是评估使用 SOV 结合微创胚胎采集技术为巴西动物基因库提供特定感兴趣基因型种质的可行性和效率。Morada Nova（ = 20）、Santa Inês（ = 20）和 Somalis（ = 20）母羊接受阴道内孕酮（330 mg）装置 9 天，而 Canindé（ = 15）和 Moxotó（ = 15）山羊接受阴道内醋酸甲羟孕酮（60 mg）装置 6 天。所有雌性动物在撤去装置前 60 小时开始，每隔 12 小时接受 6 次递减剂量的 133 mg 猪促卵泡素（pFSH），外加第 5 和第 6 次 pFSH 剂量时给予 37.5 μg 的 d-氯前列烯醇。发情的供体与可育雄性动物交配。在 NSER 前 1 天通过超声检查评估黄体（CL）数量。发情后第 6.5 或第 7 天，在激素诱导的宫颈松弛后进行 NSER。97%的绵羊和 90%的山羊对发情有反应，其中 91%的绵羊和 85%的山羊出现了 CL。在母羊中，Santa Inês 品种的 CL 数量更大（ < 0.05），而山羊品种的 CL 数量相似（ > 0.05）。所有可存活的胚胎均可冷冻（优秀和良好质量），每只供体的数量为绵羊 7.8 个，山羊 4.9 个。绵羊和山羊的每个物种中，NSER 效率、胚胎产量和 NSER 后生育力的所有参数均无差异（ > 0.05）。应用于本地适应的巴西小反刍动物品种胚胎生物库供应的 SOV-NSER 程序在生产可冷冻胚胎和保存供体生育力方面均有效。因此，建议在绵羊和山羊中使用 SOV 后进行 NSER 来组装胚胎生物库。
