Co-transfection with and via chitosan nanoparticles potentiates osteogenesis in human adipose-derived stromal cells .

OBJECTIVE

To investigate if co-transfection of human bone morphogenetic protein 2 (BMP-2, ) and human fibroblast growth factor 2 (FGF2, ) via chitosan nanoparticles promotes osteogenesis in human adipose tissue-derived stem cells (ADSCs) .

MATERIALS AND METHODS

Recombinant and/or expression vectors were constructed and packaged into chitosan nanoparticles. The chitosan nanoparticles were characterized by atomic force microscopy. Gene and protein expression levels of BMP-2 and FGF2 in ADSCs were evaluated by real-time polymerase chain reaction (PCR), western blot, and enzyme-linked immunosorbent assay. Osteocalcin (OCN) and bone sialoprotein (BSP) gene expression were also evaluated by real-time PCR to assess osteogenesis.

RESULTS

The prepared chitosan nanoparticles were spherical with a relatively homogenous size distribution. The and vectors were successfully transfected into ADSCs. BMP-2 and FGF2 mRNA and protein levels were significantly up-regulated in the co-transfection group compared with the control group. OCN and BSP mRNA levels were also significantly increased in the co-transfection group compared with cells transfected with or alone, suggesting that co-transfection significantly enhanced osteogenesis.

CONCLUSIONS

Co-transfection of human ADSCs with via chitosan nanoparticles efficiently promotes the osteogenic properties of ADSCs .