• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

BMP2和BMP7基因修饰的大鼠脂肪来源干细胞构建组织工程骨的成骨研究

The osteogenic study of tissue engineering bone with BMP2 and BMP7 gene-modified rat adipose-derived stem cell.

作者信息

Qing Wang, Guang-Xing Chen, Lin Guo, Liu Yang

机构信息

Center for Joint Surgery, South West Hospital, Third Military Medical University, Chongqing 400038, China.

出版信息

J Biomed Biotechnol. 2012;2012:410879. doi: 10.1155/2012/410879. Epub 2012 Jun 21.

DOI:10.1155/2012/410879
PMID:22778550
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3388521/
Abstract

To evaluate the feasibility and advantages of constructing a novel tissue engineering bone, using β-tricalcium phosphate (β-TCP) and rat adipose-derived stem cells (ADSCs), modified with BMP2 and BMP7 by lentivirus. In the present study, ADSCs transfected with Lv-BMP2 and Lv-BMP7, alone or together, were seeded on β-TCP scaffold and cultured in vitro. Based on the results of DNA assay, alkaline phosphatase (ALP) activity, alizarin red staining and osteogenic marker genes expression analysis, the BMP2 and BMP7 genes cotransfection group exhibited a higher degree of osteogenic differentiation in vitro. To investigate the in vivo osteogenesis of the tissue engineering bone, the ADSCs/β-TCP constructs were implanted in rat femurs defects for 6 weeks and studied histomorphology and radiography. The results showed that BMP2 and BMP7 genes cotransfection group dramatically enhanced the efficiency of new bone formation than BMP2 group and BMP7 group in vivo. These results demonstrated that it was advantageous to construct tissue engineering bone using ADSCs cotransfected with BMP2 and BMP7 on β-TCP, providing a potential way for treating bone defects.

摘要

为评估利用β-磷酸三钙（β-TCP）和经慢病毒转染BMP2和BMP7修饰的大鼠脂肪来源干细胞（ADSCs）构建新型组织工程骨的可行性和优势。在本研究中，将单独或共同用Lv-BMP2和Lv-BMP7转染的ADSCs接种于β-TCP支架上并进行体外培养。基于DNA检测、碱性磷酸酶（ALP）活性、茜素红染色及成骨标记基因表达分析结果，BMP2和BMP7基因共转染组在体外表现出更高程度的成骨分化。为研究组织工程骨的体内成骨情况，将ADSCs/β-TCP构建体植入大鼠股骨缺损处6周，并进行组织形态学和影像学研究。结果表明，在体内，BMP2和BMP7基因共转染组比BMP2组和BMP7组显著提高了新骨形成效率。这些结果表明，用BMP2和BMP7共转染的ADSCs在β-TCP上构建组织工程骨具有优势，为治疗骨缺损提供了一种潜在方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e827/3388521/363dd3437763/JBB2012-410879.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e827/3388521/79a0106625d6/JBB2012-410879.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e827/3388521/aa8f5c28bd54/JBB2012-410879.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e827/3388521/7e2411bc877d/JBB2012-410879.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e827/3388521/8cb58eb2545b/JBB2012-410879.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e827/3388521/50ab099a5839/JBB2012-410879.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e827/3388521/363dd3437763/JBB2012-410879.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e827/3388521/79a0106625d6/JBB2012-410879.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e827/3388521/aa8f5c28bd54/JBB2012-410879.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e827/3388521/7e2411bc877d/JBB2012-410879.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e827/3388521/8cb58eb2545b/JBB2012-410879.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e827/3388521/50ab099a5839/JBB2012-410879.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e827/3388521/363dd3437763/JBB2012-410879.006.jpg

相似文献

1
The osteogenic study of tissue engineering bone with BMP2 and BMP7 gene-modified rat adipose-derived stem cell.BMP2和BMP7基因修饰的大鼠脂肪来源干细胞构建组织工程骨的成骨研究
J Biomed Biotechnol. 2012;2012:410879. doi: 10.1155/2012/410879. Epub 2012 Jun 21.
2
[The feasibility of adenoviral co-transduction of BMP2 and BMP7 for the expression of recombinant human BMP2/7 heterodimer in rat bone marrow mesenchymal stem cells].腺病毒共转导BMP2和BMP7在大鼠骨髓间充质干细胞中表达重组人BMP2/7异二聚体的可行性
Zhonghua Zheng Xing Wai Ke Za Zhi. 2016 Mar;32(2):125-29.
3
Reprogramming of mesenchymal stem cells derived from iPSCs seeded on biofunctionalized calcium phosphate scaffold for bone engineering.将 iPSCs 来源的间充质干细胞种植在生物功能化的磷酸钙支架上进行骨工程的重编程。
Biomaterials. 2013 Oct;34(32):7862-72. doi: 10.1016/j.biomaterials.2013.07.029. Epub 2013 Jul 24.
4
Effect of BMPs and Wnt3a co-expression on the osteogenetic capacity of osteoblasts.骨形态发生蛋白（BMPs）与Wnt3a共表达对成骨细胞成骨能力的影响。
Mol Med Rep. 2016 Nov;14(5):4328-4334. doi: 10.3892/mmr.2016.5734. Epub 2016 Sep 12.
5
Human Urine Derived Stem Cells in Combination with β-TCP Can Be Applied for Bone Regeneration.人尿源干细胞与β-磷酸三钙联合应用可用于骨再生。
PLoS One. 2015 May 13;10(5):e0125253. doi: 10.1371/journal.pone.0125253. eCollection 2015.
6
Effect of CGRP-adenoviral vector transduction on the osteoblastic differentiation of rat adipose-derived stem cells.降钙素基因相关肽腺病毒载体转导对大鼠脂肪源性干细胞成骨分化的影响。
PLoS One. 2013 Aug 30;8(8):e72738. doi: 10.1371/journal.pone.0072738. eCollection 2013.
7
The interactions between rat-adipose-derived stromal cells, recombinant human bone morphogenetic protein-2, and beta-tricalcium phosphate play an important role in bone tissue engineering.大鼠脂肪来源基质细胞、重组人骨形态发生蛋白-2 和 β-磷酸三钙之间的相互作用在骨组织工程中起着重要作用。
Tissue Eng Part A. 2010 Sep;16(9):2927-40. doi: 10.1089/ten.TEA.2010.0018.
8
[RECOMBINANT ADENOVIRUS-MEDIATED BONE MORPHOGENETIC PROTEIN 9 AND ERYTHROPOIETIN GENES CO-TRANSFECTION IN PROMOTING OSTEOGENIC DIFFERENTIATION OF ADIPOSE-DERIVED STEM CELLS IN VITRO].[重组腺病毒介导骨形态发生蛋白9与促红细胞生成素基因共转染促进脂肪干细胞体外成骨分化]
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2016 Mar;30(3):272-8.
9
Experimental construction of BMP2 and VEGF gene modified tissue engineering bone in vitro.BMP2与VEGF基因修饰组织工程骨的体外实验构建
Int J Mol Sci. 2011;12(3):1744-55. doi: 10.3390/ijms12031744. Epub 2011 Mar 7.
10
MgO-enhanced β-TCP promotes osteogenesis in both in vitro and in vivo rat models.氧化镁增强的 β-TCP 在体外和体内大鼠模型中均促进成骨。
Sci Rep. 2024 Aug 25;14(1):19725. doi: 10.1038/s41598-024-70512-5.

引用本文的文献

1
Expression of Osteogenic Genes in Dental Pulp and Their Correlations in Patients with Clinically Diagnosed Irreversible Pulpitis.临床诊断为不可复性牙髓炎患者牙髓中成骨基因的表达及其相关性
Eur Endod J. 2025 May;10(3):198-204. doi: 10.14744/eej.2025.24008.
2
Regional Gene Therapy for Bone Tissue Engineering: A Current Concepts Review.骨组织工程的区域基因治疗：当前概念综述
Bioengineering (Basel). 2025 Jan 27;12(2):120. doi: 10.3390/bioengineering12020120.
3
Effect of Porcine- and Bovine-Derived Xenografts with Hydroxypropyl Methylcellulose for Bone Formation in Rabbit Calvaria Defects.

本文引用的文献

1
Transplantation of Cbfa1-overexpressing adipose stem cells together with vascularized periosteal flaps repair segmental bone defects.过表达 Cbfa1 的脂肪干细胞与带血管化骨膜瓣联合移植修复节段性骨缺损。
J Surg Res. 2012 Jul;176(1):e13-20. doi: 10.1016/j.jss.2011.12.011. Epub 2012 Jan 2.
2
Low-dose rhBMP2/7 heterodimer to reconstruct peri-implant bone defects: a micro-CT evaluation.低剂量 rhBMP2/7 杂二聚体重建种植体周围骨缺损：一项 micro-CT 评估。
J Clin Periodontol. 2012 Jan;39(1):98-105. doi: 10.1111/j.1600-051X.2011.01807.x. Epub 2011 Nov 10.
3
Over-expression of VEGF165 in the adipose tissue-derived stem cells via the lentiviral vector.
猪源和牛源异种移植物与羟丙基甲基纤维素对兔颅骨缺损骨形成的影响
Materials (Basel). 2023 Feb 23;16(5):1850. doi: 10.3390/ma16051850.
4
A bone-on-a-chip collagen hydrogel-based model using pre-differentiated adipose-derived stem cells for personalized bone tissue engineering.基于骨芯片胶原水凝胶的模型，使用预分化的脂肪来源干细胞进行个性化骨组织工程。
J Biomed Mater Res A. 2023 Jan;111(1):88-105. doi: 10.1002/jbm.a.37448. Epub 2022 Oct 7.
5
Surgical Classification for Preclinical Rat Femoral Bone Defect Model: Standardization Based on Systematic Review, Anatomical Analysis and Virtual Surgery.临床前大鼠股骨骨缺损模型的手术分类：基于系统评价、解剖学分析和虚拟手术的标准化
Bioengineering (Basel). 2022 Sep 15;9(9):476. doi: 10.3390/bioengineering9090476.
6
BMP-2 Enhances Osteogenic Differentiation of Human Adipose-Derived and Dental Pulp Stem Cells in 2D and 3D Models.BMP-2在二维和三维模型中增强人脂肪来源干细胞和牙髓干细胞的成骨分化
Stem Cells Int. 2022 Mar 4;2022:4910399. doi: 10.1155/2022/4910399. eCollection 2022.
7
Current evidence on potential of adipose derived stem cells to enhance bone regeneration and future projection.脂肪来源干细胞促进骨再生潜力的当前证据及未来展望。
World J Stem Cells. 2021 Sep 26;13(9):1248-1277. doi: 10.4252/wjsc.v13.i9.1248.
8
The Role of Adipose Stem Cells in Bone Regeneration and Bone Tissue Engineering.脂肪干细胞在骨再生和骨组织工程中的作用。
Cells. 2021 Apr 21;10(5):975. doi: 10.3390/cells10050975.
9
Constructing a Sr-Substituted Surface Hydroxyapatite Hexagon-Like Microarray on 3D-Plotted Hydroxyapatite Scaffold to Regulate Osteogenic Differentiation.在三维打印羟基磷灰石支架上构建锶取代的表面羟基磷灰石六边形微阵列以调节成骨分化。
Nanomaterials (Basel). 2020 Aug 26;10(9):1672. doi: 10.3390/nano10091672.
10
Recent Advances in Biomaterials for the Treatment of Bone Defects.生物材料在骨缺损治疗中的最新进展。
Organogenesis. 2020 Oct 1;16(4):113-125. doi: 10.1080/15476278.2020.1808428. Epub 2020 Aug 16.
通过慢病毒载体在脂肪组织来源的干细胞中过表达 VEGF165。
Chin Med J (Engl). 2011 Oct;124(19):3093-7.
4
[Expression of human bone morphogenetic protein 7 gene in adipose-derived stem cells and its effects on osteogenic phenotype].人骨形态发生蛋白7基因在脂肪来源干细胞中的表达及其对成骨表型的影响
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2011 Jul;25(7):848-53.
5
Experimental construction of BMP2 and VEGF gene modified tissue engineering bone in vitro.BMP2与VEGF基因修饰组织工程骨的体外实验构建
Int J Mol Sci. 2011;12(3):1744-55. doi: 10.3390/ijms12031744. Epub 2011 Mar 7.
6
Bone formation and degradation of a highly porous biphasic calcium phosphate ceramic in presence of BMP-7, VEGF and mesenchymal stem cells in an ectopic mouse model.在异位小鼠模型中，BMP-7、VEGF 和间充质干细胞存在的情况下，高度多孔双相磷酸钙陶瓷的骨形成和降解。
J Craniomaxillofac Surg. 2010 Sep;38(6):423-30. doi: 10.1016/j.jcms.2010.01.003. Epub 2010 Mar 1.
7
Osteogenic induction of adipose-derived stromal cells: not a requirement for bone formation in vivo.脂肪来源基质细胞的成骨诱导：体内骨形成并非必需。
Artif Organs. 2010 Jan;34(1):46-54. doi: 10.1111/j.1525-1594.2009.00795.x. Epub 2009 Oct 11.
8
Hyaluronan benzyl ester as a scaffold for tissue engineering.透明质酸苄酯作为组织工程支架。
Int J Mol Sci. 2009 Jul 3;10(7):2972-2985. doi: 10.3390/ijms10072972.
9
Combinatorial gene therapy with BMP2/7 enhances cranial bone regeneration.BMP2/7联合基因疗法可增强颅骨再生。
J Dent Res. 2008 Sep;87(9):845-9. doi: 10.1177/154405910808700906.
10
Defining stem and progenitor cells within adipose tissue.定义脂肪组织中的干细胞和祖细胞。
Stem Cells Dev. 2008 Dec;17(6):1053-63. doi: 10.1089/scd.2008.0117.