从根部分离得到的三种新的倍半萜生物碱及其细胞毒性。

Three new sesquiterpenoid alkaloids from the roots of and its cytotoxicity.

机构信息

Key Laboratory of Chinese Materia Medica, Heilongjiang University of Chinese Medicine, Ministry of Education, Harbin, People's Republic of China.

出版信息

Nat Prod Res. 2022 Aug;36(15):3979-3987. doi: 10.1080/14786419.2021.1903460. Epub 2021 Mar 26.

DOI:10.1080/14786419.2021.1903460

PMID:33769155

Abstract

Three new sesquiterpenoid alkaloids, cangorin K (), dimacroregelines C () and D (), as well as two known sesquiterpenoids (-), were isolated from the roots of The structures of new compounds were characterised by extensive 1D and 2D NMR spectroscopic analyses, as well as HRESIMS data, and the known compounds were established by 1 D NMR spectra referring to the literatures. Cytotoxicity evaluation of these compounds against two human tumour lines (SMMC7721, LN229) was investigated by CCK-8 assay and displayed that compounds - showed potent cytotoxicity against SMMC7721 cell with IC value in the range of 0.26-9.67 μΜ and compounds - showed potent cytotoxicity against LN-229 cell with IC values in the range of 0.50-7.38 μΜ.

摘要

从根部分离得到三个新的倍半萜生物碱，苍果因 K ( )、二甲基异喹啉 C () 和 D ()，以及两个已知的倍半萜生物碱 (-)。新化合物的结构通过广泛的 1D 和 2D NMR 光谱分析以及 HRESIMS 数据进行了表征，而已知化合物则通过参考文献的 1D NMR 光谱进行了确定。通过 CCK-8 测定法评估了这些化合物对两种人类肿瘤细胞系（SMMC7721、LN229）的细胞毒性，结果表明，化合物 - 对 SMMC7721 细胞具有很强的细胞毒性，IC 值范围为 0.26-9.67 μM，化合物 - 对 LN-229 细胞具有很强的细胞毒性，IC 值范围为 0.50-7.38 μM。