Laboratorio de Biología Molecular, Departamento de Ingeniería Bioquímica, Tecnológico Nacional de México/ITCelaya, Celaya, México.
Unidad de Genómica Avanzada (UGA-LANGEBIO), Centro De Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Irapuato, México.
Plant Signal Behav. 2021 Jun 3;16(6):1905335. doi: 10.1080/15592324.2021.1905335. Epub 2021 Mar 26.
Cysteine-rich receptor-like protein kinases (CRKs) are transmembrane proteins containing two domains of unknown function 26 (DUF26) RLKs in their ectodomain. Despite that CRKs control important aspects of plant development, only few proteins have functionally been characterized. In this work, we analyzed the function of CRK33 by characterizing two insertional lines. The stomatal density and stomatal index were decreased in and plants in comparison to wild-type plants, correlating with a decreased transpiration in transgenic plants and a higher drought tolerance. Furthermore, photosynthesis and stomatal conductance changed. Finally, all four stomata cell fate genes were upregulated, especially the expression of and in the mutant background, suggesting a role for CRK33 in stomatal spacing.
富含半胱氨酸的受体样蛋白激酶（CRKs）是一种跨膜蛋白，其细胞外域含有两个功能未知的域 26（DUF26）RLKs。尽管 CRKs 控制着植物发育的重要方面，但只有少数蛋白质具有功能特征。在这项工作中，我们通过对两个插入系进行分析来研究 CRK33 的功能。与野生型植物相比，和 植株的气孔密度和气孔指数降低，与转基因植物蒸腾作用降低和耐旱性提高相关。此外，光合作用和气孔导度也发生了变化。最后，所有四个气孔细胞命运基因都上调，特别是在突变背景下 和 的表达，这表明 CRK33 可能在气孔间距中起作用。
