Key Laboratory of Huang-Huai-Hai Smart Agricultural Technology of the Ministry of Agriculture and Rural Affairs, College of Information Science and Engineering, Shandong Agricultural University, Tai'an, Shandong, 271018, People's Republic of China.
College of Agronomy, Liaocheng University, Liaocheng, 252059, People's Republic of China.
BMC Genomics. 2023 May 1;24(1):224. doi: 10.1186/s12864-023-09303-7.
The receptor-like kinase (RLK) gene families in plants contains a large number of members. They are membrane proteins with an extracellular receptor domain and participate in biotic and abiotic stress responses.
In this study, we identified RLKs in 15 representative plant genomes, including wheat, and classified them into 64 subfamilies by using four types of phylogenetic trees and HMM models. Conserved exon‒intron structures with conserved exon phases in the kinase domain were found in many RLK subfamilies from Physcomitrella patens to Triticum aestivum. Domain distributions of RLKs were also diagrammed. Collinearity events and tandem gene clusters suggested that polyploidization and tandem duplication events contributed to the member expansions of T. aestivum RLKs. Global expression pattern analysis was performed by using public transcriptome data. These analyses were involved in T. aestivum, Aegilops tauschii and Brachypodium distachyon RLKs under biotic and abiotic stresses. We also selected 9 RLKs to validate the transcriptome prediction by using qRT‒PCR under drought treatment and with Fusarium graminearum infection. The expression trends of these 9 wheat RLKs from public transcriptome data were consistent with the results of qRT‒PCR, indicating that they might be stress response genes under drought or F. graminearum treatments.
In this study, we identified, classified, evolved, and expressed RLKs in wheat and related plants. Thus, our results will provide insights into the evolutionary history and molecular mechanisms of wheat RLKs.
植物中的受体样激酶（RLK）基因家族包含大量成员。它们是具有细胞外受体结构域的膜蛋白，参与生物和非生物胁迫反应。
本研究在包括小麦在内的 15 个代表性植物基因组中鉴定了 RLKs，并使用四种系统发育树和 HMM 模型将其分为 64 个亚家族。在从石松属到小麦属的许多 RLK 亚家族中发现了具有激酶结构域保守外显子-内含子结构和保守外显子相位的 RLKs。还绘制了 RLKs 的结构域分布。共线性事件和串联基因簇表明，多倍化和串联重复事件导致了小麦 RLKs 成员的扩张。通过使用公共转录组数据进行了全球表达模式分析。这些分析涉及生物和非生物胁迫下的小麦、节节麦和短柄草的 RLKs。我们还选择了 9 个 RLKs，通过 qRT-PCR 在干旱处理和感染禾谷镰刀菌时验证转录组预测。这 9 个小麦 RLKs 的表达趋势与 qRT-PCR 的结果一致，表明它们可能是干旱或禾谷镰刀菌处理下的胁迫反应基因。
本研究在小麦和相关植物中鉴定、分类、进化和表达了 RLKs。因此，我们的结果将为小麦 RLKs 的进化历史和分子机制提供深入了解。
