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氢氧化钙盖髓后基于环氧树脂的封闭剂渗入牙本质小管的共聚焦激光扫描显微镜分析

Confocal laser scanning microscopic analysis of the penetration of an epoxy resin-based sealer into dentinal tubules after calcium hydroxide dressing.

作者信息

Machado Manoel E L, Veintimilla Lozada Virginia Natalia, Carrillo Rengifo Karol Jasmin, Guillén Raquel E G, Caballero-Flores Hector, Nabeshima Cleber Keiti

机构信息

Department of Restorative Dentistry, School of Dentistry, University of São Paulo, São Paulo, Brazil.

School of Dentistry, University Central del Ecuador, Quito, Ecuador.

出版信息

Aust Endod J. 2021 Dec;47(3):480-486. doi: 10.1111/aej.12508. Epub 2021 Mar 26.

DOI:10.1111/aej.12508
PMID:33769648
Abstract

This study assessed the penetration of an epoxy resin-based sealer into dentinal tubules of root canals previously medicated with calcium hydroxide. Sixteen palatal root canals of upper molars were instrumented and distributed into two groups: (G1) root canals medicated with calcium hydroxide and obturated after its removal; (G2) root canals obturated without the use of dressing. Sealer mixed with rhodamine B provided fluorescence for confocal laser scanning microscopy. Measurements of area and linear penetration of the sealer were assessed in three thirds by ImageJ software. The data were statistically compared by Kruskal-Wallis, Dunn's and Mann-Whitney U tests (P < 0.01). The calcium hydroxide decreased the penetration of the sealer. The coronal and middle thirds had similar areas and linear penetrations in both groups, whereas the apical third had less penetration. In conclusion, the penetration of the epoxy resin-based sealer is influenced by the calcium hydroxide dressing used between appointments.

摘要

本研究评估了一种环氧树脂类封闭剂渗入先前用氢氧化钙进行过根管内药物治疗的牙本质小管的情况。对上颌磨牙的16个腭侧根管进行预备,并分为两组:(G1)用氢氧化钙进行根管内药物治疗并在去除后进行充填的根管;(G2)未使用根管内封药进行充填的根管。与罗丹明B混合的封闭剂为共聚焦激光扫描显微镜提供荧光。通过ImageJ软件在三个1/3段评估封闭剂的面积和线性渗入情况。数据通过Kruskal-Wallis检验、Dunn检验和Mann-Whitney U检验进行统计学比较(P < 0.01)。氢氧化钙降低了封闭剂的渗入。两组的冠部和中部1/3段具有相似的面积和线性渗入情况,而根尖1/3段的渗入较少。总之,环氧树脂类封闭剂的渗入受预约期间使用的氢氧化钙封药的影响。

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引用本文的文献

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BMC Oral Health. 2025 Jul 26;25(1):1253. doi: 10.1186/s12903-025-06657-0.
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Dentinal tubular penetration ability of epoxy resin-based and bioceramic-based root canal sealers.基于环氧树脂和生物陶瓷的根管封闭剂的牙本质小管渗透能力。
BMC Oral Health. 2024 Dec 19;24(1):1506. doi: 10.1186/s12903-024-05311-5.
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Evaluation of different irrigation activation techniques for the removal of various medicaments from a simulated internal resorption cavity: an in vitro study.
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