Sun Jie, Shen Fujun, Zhang Liang, Luo Li, Fan Zhenxin, Hou Rong, Yue Bisong, Zhang Xiuyue
Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, China.
The Sichuan Key Laboratory for Conservation Biology of Endangered Wildlife, Chengdu Research Base of Giant Panda Breeding, Chengdu, China.
DNA Cell Biol. 2021 Apr;40(4):595-605. doi: 10.1089/dna.2020.5942. Epub 2021 Mar 26.
Canine distemper (CD) is a significant threat to wild and captive giant panda populations. Captive giant pandas are inoculated with canine distemper virus (CDV) vaccination to prevent the infection with the CDV. As an important regulator, microRNA (miRNA) plays a crucial role in regulating gene expression, including in disease immunity. To understand the role of miRNA in immune response to CDV vaccination, we investigated the miRNA expression profile in five giant panda cubs after two inoculations, 21 days apart. A total of 187 conserved miRNAs and 96 novel miRNAs were identified. Among the 187 conserved miRNAs, 29 differentially expressed miRNAs were found postinoculation. The upregulation of miR-16, miR-182, miR-30b, and miR-101 indicated that the innate immune may be enhanced, whereas the upregulation of miR-142 and miR-19a are probably involved in the enhanced cellular immune response. However, the downregulated miR-155 and miR-181a might indicate the giant panda has weak ability to produce antibodies and memory B cells. Integrated analysis of miRNA-messenger RNA (mRNA) found 20 negatively regulated miRNA-mRNA pairs, where downregulated miR-204 might enhance giant panda cub innate immunity by increasing expression, and downregulated miR-330 might activate macrophages and regulate the immune response by increasing expression. Our research provides key information for future development to enhance the immune response of giant pandas and potentially improve the survival of captive and wild giant panda populations threatened by CD.
犬瘟热（CD）对野生和圈养大熊猫种群构成重大威胁。圈养大熊猫会接种犬瘟热病毒（CDV）疫苗以预防感染CDV。作为一种重要的调节因子，微小RNA（miRNA）在调节基因表达（包括疾病免疫中的基因表达）方面发挥着关键作用。为了解miRNA在对CDV疫苗免疫反应中的作用，我们调查了5只大熊猫幼崽在间隔21天进行两次接种后的miRNA表达谱。共鉴定出187个保守miRNA和96个新miRNA。在187个保守miRNA中，接种后发现29个差异表达的miRNA。miR-16、miR-182、miR-30b和miR-101的上调表明先天免疫可能增强，而miR-142和miR-19a的上调可能参与了细胞免疫反应的增强。然而，miR-155和miR-181a的下调可能表明大熊猫产生抗体和记忆B细胞的能力较弱。miRNA-信使核糖核酸（mRNA）的综合分析发现了20对负调控的miRNA-mRNA对，其中下调的miR-204可能通过增加 表达来增强大熊猫幼崽的先天免疫，下调的miR-330可能通过增加 表达来激活巨噬细胞并调节免疫反应。我们的研究为未来增强大熊猫免疫反应的发展提供了关键信息，并有可能提高受到CD威胁的圈养和野生大熊猫种群的存活率。
