CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, CAS Center for Excellence in Nanoscience, National Center for NanoScience and Technology, No. 11 Zhongguancun Beiyitiao, Beijing, 100190, P. R. China.
Department of Biomedical Engineering, Southern University of Science and Technology, No. 1088 Xueyuan Road, Nanshan District, Shenzhen, Guangdong, 518055, P. R. China.
Angew Chem Int Ed Engl. 2021 May 25;60(22):12319-12322. doi: 10.1002/anie.202101293. Epub 2021 Apr 26.
We herein develop a concentration gradient generator (CGG) on a microfluidic chip for diluting different nanoparticles. Specifically designed compact disk (CD)-shaped microchannels in the CGG module could thoroughly mix the flowing solutions and generate a linear concentration gradient of nanoparticles without aggregation. We combine the CGG with a single-cell trapper array (SCA) on microfluidics to evaluate the concentration-dependent bioeffects of the nanoparticles. The precise control of the spatiotemporal generation of nanoparticle concentration on the CGG module and the single-cell-level monitoring of the cell behaviors on the SCA module by a high-content system in real time, render the CGG-SCA system a highly precise platform, which can exclude the average effect of cell population and reflect the response of individual cells to the gradient concentrations accurately. In addition, the CGG-SCA system provides an automated platform for high-throughput screening of nanomedicines with high precision and low sample consumption.
我们在此开发了一种基于微流控芯片的浓度梯度发生器 (CGG)，用于稀释不同的纳米粒子。CGG 模块中专门设计的紧凑光盘 (CD) 形微通道可以彻底混合流动溶液并产生纳米粒子的线性浓度梯度，而不会发生聚集。我们将 CGG 与微流控上的单细胞捕捉器阵列 (SCA) 相结合，以评估纳米粒子的浓度依赖性生物效应。通过高内涵系统实时精确控制 CGG 模块上纳米粒子浓度的时空生成以及 SCA 模块上单细胞水平的细胞行为监测，使 CGG-SCA 系统成为一个高度精确的平台，该平台可以排除细胞群体的平均效应，并准确反映单个细胞对梯度浓度的反应。此外，CGG-SCA 系统提供了一个自动化平台，用于高精度和低样品消耗的纳米药物高通量筛选。
