School of Materials Science and Engineering, South China University of Technology, Guangzhou 510640, China.
National Engineering Research Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou 510006, China.
Int J Mol Sci. 2020 May 30;21(11):3939. doi: 10.3390/ijms21113939.
Calcium phosphates (CaP) represent an impressive kind of biomedical material due to their excellent biocompatibility, bioactivity, and biodegradability. Their morphology and structure highly influence their properties and applications. Whilst great progress has been made in research on biomedical materials, there is still a need to develop a method that can rapidly synthesize and screen micro/nanosized biomedical materials. Here, we utilized a microarray screening platform that could provide the high-throughput synthesis of biomedical materials and screen the vital reaction conditions. With this screening platform, 9 × 9 sets of parallel experiments could be conducted simultaneously with one- or two-dimensions of key reaction condition gradients. We used this platform to establish a one-dimensional gradient of the pH and citrate concentration and a two-dimensional gradient of both the Ca/P ratio and pH to synthesize CaP particles with various morphologies. This screening platform also shows the potential to be extended to other reaction systems for rapid high-throughput screening.
钙磷（CaP）因其优异的生物相容性、生物活性和可生物降解性而成为一种引人注目的生物医学材料。其形态和结构对其性能和应用有很大的影响。虽然在生物医学材料的研究方面已经取得了很大的进展，但仍需要开发一种能够快速合成和筛选微/纳米生物医学材料的方法。在这里，我们利用微阵列筛选平台，可以提供生物医学材料的高通量合成和筛选重要的反应条件。利用这个筛选平台，可以同时进行 9×9 组平行实验，具有一维或二维的关键反应条件梯度。我们使用这个平台建立了 pH 值和柠檬酸盐浓度的一维梯度以及 Ca/P 比和 pH 值的二维梯度，以合成具有各种形貌的 CaP 颗粒。该筛选平台还具有扩展到其他反应系统以进行快速高通量筛选的潜力。
