Key Laboratory of Marine Drugs, Chinese Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao, People's Republic of China.
Key Laboratory of Marine Drugs, Chinese Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao, People's Republic of China; Laboratory for Marine Drugs and Bioproducts, Pilot National Laboratory for Marine Science and Technology, Qingdao, People's Republic of China.
Curr Opin Biotechnol. 2021 Jun;69:281-289. doi: 10.1016/j.copbio.2021.02.008. Epub 2021 Mar 23.
Terpenoids, also referred to as isoprenoids, are the largest group of natural compounds which have contributed significantly to the pharmaceutical industry. The challenges in producing bioactive terpenoids from their original host or by organic synthesis methods spurred the endeavors of producing terpenoids in heterologous host. Modern advances utilizing synthetic biology and biological engineering tools have provided a variety of pharmaceutical terpenoids in large-scale and with diversified structures. In this review, we will summarize the progress in production of typical terpenoids skeletons using heterologous expression method assisted by metabolic engineering techniques, with the purpose of enlightening further efforts in developing advanced cell factories for producing terpenoid based pharmaceuticals.
萜类化合物，也被称为异戊二烯类化合物，是最大的天然化合物群体，对制药行业做出了重大贡献。从原始宿主或通过有机合成方法生产生物活性萜类化合物的挑战，促使人们在异源宿主中生产萜类化合物。现代利用合成生物学和生物工程工具的进步，以大规模和多样化的结构提供了各种药用萜类化合物。在这篇综述中，我们将总结利用代谢工程技术辅助的异源表达方法生产典型萜类骨架的进展，旨在为开发生产基于萜类的药物的先进细胞工厂提供进一步的努力。
