Key Laboratory of Crop Integrated Pest Management in South China, Ministry of Agriculture and Rural Affairs, South China Agricultural University, Guangzhou 510642, China; Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education, South China Agricultural University, Guangzhou 510642, China.
Key Laboratory of Crop Integrated Pest Management in South China, Ministry of Agriculture and Rural Affairs, South China Agricultural University, Guangzhou 510642, China; Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education, South China Agricultural University, Guangzhou 510642, China; Guangdong Research Institute of Petrochemical and Fine Chemical Engineering, Guangdong Academy of Sciences, Guangzhou 510642, China.
Pestic Biochem Physiol. 2021 Mar;173:104801. doi: 10.1016/j.pestbp.2021.104801. Epub 2021 Feb 11.
Rotenone, a selective inhibitor of mitochondrial complex I, has been extensively studied on kinds of neuron and neuroblast in Parkinson's disease. However, little is known about the potential mechanism of this promising botanical insecticide upon insect cells. In the article, cell proliferation of two Lepidoptera cell lines, Spodoptera litura SL-1 cells and Spodoptera frugiperda Sf9 cells, were all inhibited by rotenone in a time- and dose-dependent manner. Typical necrotic characteristics of cell morphology and ultrastructure, such as plasma membrane collapses and organelle lyses, were all observed by transmission electron microscope and scanning electron microscope. Moreover, irregular DNA degradation was also detected by DNA gel electrophoresis and Hoechst 33258 staining, while the typical apoptotic feature, DNA ladder, hadn't been observed. Flow cytometric analysis showed that rotenone-induced cell death of Sf9 and SL-1 cells accompanied with the plasma membrane potential depolarization and mitochondrial membrane potential reduction. Furthermore, the activity of Na-K-ATPase was detected in our study. In conclusion, rotenone could cause necrosis but not apoptosis in insect cells through a mitochondrial- and plasmic membrane-dependent pattern, which shed a light on the rotenone-induced cytotoxicity on insects.
鱼藤酮是一种线粒体复合物 I 的选择性抑制剂，已在帕金森病的各种神经元和神经母细胞瘤中进行了广泛研究。然而，关于这种有前途的植物性杀虫剂对昆虫细胞的潜在机制知之甚少。在本文中，鱼藤酮以时间和剂量依赖的方式抑制了两种鳞翅目细胞系，斜纹夜蛾 SL-1 细胞和草地贪夜蛾 Sf9 细胞的增殖。透射电子显微镜和扫描电子显微镜都观察到了典型的坏死细胞形态和超微结构特征，如质膜崩溃和细胞器溶解。此外，DNA 凝胶电泳和 Hoechst 33258 染色也检测到了不规则的 DNA 降解，而没有观察到典型的凋亡特征，即 DNA 梯。流式细胞术分析表明，鱼藤酮诱导 Sf9 和 SL-1 细胞死亡伴随着质膜电位去极化和线粒体膜电位降低。此外，我们的研究还检测了 Na-K-ATP 酶的活性。总之，鱼藤酮通过线粒体和质膜依赖性模式引起昆虫细胞坏死而不是凋亡，这为鱼藤酮对昆虫的细胞毒性提供了新的认识。
