Fu Yu, Chen Yupeng, Wang Yani, Fu Xinyi, Jin Shunda, Yi Chunyan, Bai Xue, Lu Youqing, Miao Wang, Geng Xingyu, Lu Xianli, Yan Rihui, Zhou Zhongshi, Cao Fengqin
School of Tropical Agriculture and Forestry, Hainan University, Haikou 570100, China.
Institute of Systems Medicine and Health Sciences, Hong Kong Baptist University, Hong Kong SAR 999077, China.
Int J Mol Sci. 2025 Jul 8;26(14):6556. doi: 10.3390/ijms26146556.
(Coquillett) () is a global extremely invasive quarantine pest which has a wide host range of fruits and vegetables. At present, there are a few control measures for , and deltamethrin and avermectin are commonly used. Among the hosts of , , , , var. , , and are non-favored hosts. However, it is still not clear why these hosts are non-favored and whether there are any repellent components of in these hosts. In this study, the components of these six hosts were collected from the literature, and the genes of odor and chemical sensation were determined from the genome of . After the potential relationships between these components and genes were determined by molecular docking methods, the KEGG and GO enrichment analysis of these genes was conducted, and a complex network of genes vs. components vs. Kegg pathway vs. GO terms was constructed and used to select the key components for experiments. The results show that oleanolic acid (1 mg/mL, 0.1 mg/mL, and 0.01 mg/mL), rotenone (1 mg/mL, 0.1 mg/mL, and 0.01 mg/mL), and beta-caryophyllene oxide (1 mg/mL, 0.1 mg/mL, and 0.01 mg/mL) had a significant repellent effect on , and three components, rotenone (1 mg/mL and 0.1 mg/mL), echinocystic acid (1 mg/mL, 0.1 mg/mL, and 0.01 mg/mL), and beta-caryophyllene oxide (1 mg/mL, and 0.1 mg/mL) had significant stomach toxicity in . Furthermore, a complex signaling pathway was built and used to predict the effect of these components on . These components probably play roles in the neuroactive ligand-receptor interaction (ko04080) and calcium signaling (ko04020) pathways. This study provides a reference for the prevention and control of and a scientific reference for the rapid screening and development of new pest control drugs.
（库蚊）是一种全球极具入侵性的检疫性害虫，其寄主范围广泛，涵盖多种水果和蔬菜。目前，针对库蚊的防治措施有限，常用的有溴氰菊酯和阿维菌素。在库蚊的寄主中，[具体寄主名称1]、[具体寄主名称2]、[具体寄主名称3]、[具体寄主名称4]变种、[具体寄主名称5]、[具体寄主名称6]和[具体寄主名称7]是非适宜寄主。然而，目前尚不清楚为何这些寄主是非适宜的，以及这些寄主中是否存在库蚊的驱避成分。在本研究中，从文献中收集了这六种寄主的成分，并从库蚊基因组中确定了气味和化学感知相关基因。通过分子对接方法确定这些成分与基因之间的潜在关系后，对这些基因进行了KEGG和GO富集分析，并构建了基因-成分-KEGG通路-GO术语的复杂网络，用于筛选关键成分进行实验。结果表明，齐墩果酸（1mg/mL、0.1mg/mL和0.01mg/mL）、鱼藤酮（1mg/mL、0.1mg/mL和0.01mg/mL）和氧化石竹烯（1mg/mL、0.1mg/mL和0.01mg/mL）对库蚊具有显著的驱避作用，鱼藤酮（1mg/mL和0.1mg/mL）、echinocystic acid（1mg/mL、0.1mg/mL和0.01mg/mL）和氧化石竹烯（1mg/mL和0.1mg/mL）这三种成分对库蚊具有显著的胃毒作用。此外，构建了一个复杂的信号通路来预测这些成分对库蚊的影响。这些成分可能在神经活性配体-受体相互作用（ko04080）和钙信号传导（ko04020）通路中发挥作用。本研究为库蚊的防治提供了参考，也为新型害虫防治药物的快速筛选和开发提供了科学依据。
