Zhang Cheng, Liang Yu, Zhang Chun-Dong, Pei Jun-Peng, Wu Kun-Zhe, Li Yong-Zhi, Dai Dong-Qiu
Department of Gastrointestinal Surgery, the Fourth Affiliated Hospital of China Medical University, Shenyang 110032, China.
Department of Gastrointestinal Surgery, the Fourth Affiliated Hospital of China Medical University, Shenyang 110032, China; Department of Gastrointestinal Surgery, Graduate School of Medicine, the University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Genomics. 2021 May;113(3):1504-1513. doi: 10.1016/j.ygeno.2021.03.027. Epub 2021 Mar 24.
LncRNAs play a vital role in the tumorigenesis of gastric cancer (GC). This study determined that LINC01235 expression has greater fold changes by analyzing TCGA RNA-Seq data. The qRT-PCR assay confirmed that LINC01235 is significantly over-expressed in GC cells and tissues. Additionally, the overall survival analysis showed that patients with a higher LINC01235 expression had a poorer prognosis than those with a lower LINC01235 expression. Univariate Cox regression analysis indicated that high LINC01235 expression is positively correlated with poor prognosis. Moreover, LINC01235 was an independent poor prognostic marker for GC in multivariate Cox analysis. Invitro assays suggested that LINC01235 knockdown suppresses GC cell migration and invasion. GSEA revealed that high LINC01235 expression is strongly enriched in the EMT pathway. Western blotting results revealed that LINC01235 silencing decreases the expression of EMT-induced proteins. In conclusion, LINC01235 can promote GC cell metastasis via EMT and function as a prognostic biomarker.
长链非编码RNA（lncRNAs）在胃癌（GC）的肿瘤发生过程中起着至关重要的作用。本研究通过分析TCGA RNA测序数据确定LINC01235的表达具有更大的倍数变化。qRT-PCR检测证实LINC01235在GC细胞和组织中显著过表达。此外，总生存分析表明，LINC01235表达较高的患者预后比LINC01235表达较低的患者更差。单因素Cox回归分析表明，LINC01235高表达与不良预后呈正相关。此外，在多因素Cox分析中，LINC01235是GC的独立不良预后标志物。体外实验表明，敲低LINC01235可抑制GC细胞的迁移和侵袭。基因集富集分析（GSEA）显示，LINC01235高表达在上皮-间质转化（EMT）途径中高度富集。蛋白质免疫印迹结果显示，沉默LINC01235可降低EMT诱导蛋白的表达。总之，LINC01235可通过EMT促进GC细胞转移，并作为一种预后生物标志物发挥作用。
