Bio-Veterinary Science Program, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand; Department of Parasitology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.
Department of Parasitology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.
Parasitol Int. 2021 Aug;83:102337. doi: 10.1016/j.parint.2021.102337. Epub 2021 Mar 23.
Both Cryptosporidium spp. and Giardia duodenalis are enteric protozoan parasites that infect a wide variety of domestic animals as well as humans worldwide, causing diarrheal diseases. Giardia duodenalis assemblages C and D are specific to canine hosts and zoonotic assemblages A and B are also found in dogs as a reservoir host. In dogs, Cryptosporidium canis is the host-specific species while humans are infected by C. hominis and C. parvum and at least another 16 zoonotic Cryptosporidium species have been reported causing human infections, with C. meleagridis, C. viatorum, and C. ubiquitum being the most frequent. The objective of this study was to determine the prevalence of Cryptosporidium spp. and G. duodenalis from stray dogs in areas of Bangkok and to identify the species and assemblages. Fecal samples (540) were collected from dogs residing in 95 monasteries in 48 districts in the Bangkok metropolitan area. Nested Polymerase Chain Reaction (PCR) was performed using the ssu-rRNA gene for both parasites. In total, 3.0% (16/540) samples were positive for G. duodenalis, with most being G. duodenalis assemblage D (7/16) followed by assemblage C (7/16) and zoonotic assemblage A (2/16). The prevalence of Cryptosporidium spp. was 0.7% (4/540) based on the PCR results and all were the dog genotype C. canis. These results indicated that dogs residing in Bangkok monasteries poses a limited role as source of human giardiosis and cryptosporidiosis.
隐孢子虫属和贾第虫属都是肠道原生动物寄生虫，可感染全球范围内的各种家畜和人类，引起腹泻病。贾第虫属 C 和 D 组合专门感染犬宿主，而 A 和 B 组合也在犬中作为储存宿主存在。在犬中，隐孢子虫属犬种是宿主特异性物种，而人类则感染人隐孢子虫属和人微小隐孢子虫，至少还有另外 16 种人兽共患隐孢子虫物种被报道可引起人类感染，其中 C. meleagridis、C. viatorum 和 C. ubiquitum 最为常见。本研究的目的是确定曼谷地区流浪犬中隐孢子虫属和贾第虫属的流行情况，并鉴定其种类和组合。从曼谷大都市区 48 个区的 95 所寺院中采集了 540 份犬粪便样本。使用 ssu-rRNA 基因对这两种寄生虫进行巢式聚合酶链反应（PCR）。总共，3.0%（16/540）的样本对贾第虫属呈阳性，其中大多数是贾第虫属 D 组合（7/16），其次是 C 组合（7/16）和人兽共患 A 组合（2/16）。基于 PCR 结果，隐孢子虫属的流行率为 0.7%（4/540），全部为犬基因型 C. canis。这些结果表明，居住在曼谷寺院的犬在人类贾第虫病和隐孢子虫病的传染源方面作用有限。
