Kamyingkird Ketsarin, Phoosangwalthong Pornkamol, Klinkaew Nutsuda, Leelanupat Alisara, Kengradomkij Chanya, Chimnoi Wissanuwat, Rungnirundorn Teerapat, Nimsuphan Burin, Inpankaew Tawin
Department of Parasitology, Faculty of Veterinary Medicine, Kasetsart University, Lad Yao, Chatuchak, Bangkok 10900, Thailand.
Exotic Clinic, Kasetsart University Veterinary Teaching Hospital, Chatuchak, Bangkok 10900, Thailand.
Animals (Basel). 2022 Jul 31;12(15):1949. doi: 10.3390/ani12151949.
is a flagellate protozoa that can be transmitted via direct contact and by consuming contaminated water. It is pathogenic in humans and various other animals, including exotic pets. Pet prairie dogs are popular in Thailand, but they have not been investigated regarding giardiasis. infection was measured, and genetic characterization was performed to investigate the zoonotic potential of carried by pet prairie dogs. In total, 79 fecal samples were examined from prairie dogs visiting the Kasetsart University Veterinary Teaching Hospital during 2017-2021. Simple floatation was conducted. Two -positive samples were submitted for DNA extraction, PCR targeting the , and genes was performed, and genetic characterization using sequencing analysis was conducted. Risk factors associated with infection were analyzed. infection was found in 11 out of the 79 pet prairie dogs (13.9%). infection was significantly higher in male prairie dogs ( = 0.0345). Coccidia cysts (12.7%), the eggs of nematodes (6.3%), and amoeba cysts (2.5%) were also detected. Genetic characterization of the two -positive samples revealed that they were assemblage A, sub-genotypes AI and assemblage B, and sub-genotype BIV, the zoonotic assemblages. This was the first report of infection in pet prairie dogs in Bangkok, Thailand. The results revealed that these pet prairie dogs in Thailand were infected with zoonotic assemblages of sub-genotype AI, which might have been derived from animal contaminants, whereas sub-genotype BIV might have been derived from human contaminants. Owners of prairie dogs might be at risk of giardiasis or be the source of infection to their exotic pets.
是一种鞭毛虫原生动物，可通过直接接触和饮用受污染的水传播。它对人类和包括外来宠物在内的各种其他动物具有致病性。宠物草原犬鼠在泰国很受欢迎，但尚未对其贾第虫病进行调查。测量了感染情况，并进行了基因特征分析，以研究宠物草原犬鼠携带的贾第虫的人畜共患病潜力。2017年至2021年期间，共检查了79份来自访问Kasetsart大学兽医教学医院的草原犬鼠的粪便样本。进行了简单的漂浮法。将两份阳性样本送去进行DNA提取，针对贾第虫的、和基因进行了PCR，并使用测序分析进行了基因特征分析。分析了与贾第虫感染相关的危险因素。在79只宠物草原犬鼠中有11只（13.9%）被发现感染了贾第虫。雄性草原犬鼠的贾第虫感染率显著更高（=0.0345）。还检测到了球虫囊肿（12.7%）、线虫卵（6.3%）和阿米巴囊肿（2.5%）。对两份阳性样本的基因特征分析表明，它们分别是A群，AI亚基因型和B群，BIV亚基因型，即人畜共患群。这是泰国曼谷宠物草原犬鼠感染贾第虫的首次报告。结果表明，泰国的这些宠物草原犬鼠感染了AI亚基因型的人畜共患贾第虫群，可能源自动物污染物，而BIV亚基因型可能源自人类污染物。草原犬鼠的主人可能有感染贾第虫病的风险，或者是其外来宠物的感染源。
