Department of Epidemiology, College of Public Health and Health Professions and College of Medicine, University of Florida, Gainesville, FL, USA.
Emerging Pathogens Institute, University of Florida, Gainesville, FL, USA.
Cancer Causes Control. 2021 Jul;32(7):681-692. doi: 10.1007/s10552-021-01420-6. Epub 2021 Mar 27.
We examined gut microbiome (GM) profiles in relation to mammographic breast density (BD) and body mass index (BMI) in healthy postmenopausal women.
Eligible women were postmenopausal, had a BMI ≤ 35 kg/m, and had not recently taken oral/IV antibiotics. All women provided a fecal sample and information on breast cancer risk factors. Mammographic BD was classified with the American College of Radiology's BI-RADS BD classification system. Bacterial DNA was isolated from fecal samples and the V1-V2 hypervariable regions of 16S rRNA were sequenced on the Illumina MiSeq platform. We examined associations of GM with indices of within-sample (alpha) diversity and the ratio of the two main phyla (Firmicutes and Bacteroidetes; F/B ratio) with BD and BMI.
Among 69 women with BD data, 39 had low BD (BI-RADS I/II) and 30 had high BD (BI-RADS III/IV). BMI was inversely associated with BD (mean BMI = 23.8 and 28.0 in women with high and low BD, respectively, p = 1.07 × 10). Similar levels of GM diversity were found across weight groups according to Shannon (p = 0.83); Inverse Simpson (p = 0.97); and Chao1 (p = 0.31) indices. F/B ratio and microbiota diversity were suggestively greater in women with high vs. low BD (p = 0.35, 0.14, 0.15, and 0.17 for F/B ratio, Shannon, Inverse Simpson and Chao1, respectively).
Suggestive differences observed in women with high and low BD with respect to GM alpha diversity and prevalence of specific GM taxa need to be confirmed in larger studies.
我们研究了肠道微生物组（GM）与健康绝经后女性的乳房密度（BD）和体重指数（BMI）之间的关系。
符合条件的女性为绝经后女性，BMI≤35kg/m，最近未服用口服/静脉用抗生素。所有女性均提供粪便样本，并提供乳腺癌危险因素信息。乳房密度使用美国放射学院的 BI-RADS 密度分类系统进行分类。从粪便样本中分离细菌 DNA，并在 Illumina MiSeq 平台上对 16S rRNA 的 V1-V2 高变区进行测序。我们研究了 GM 与样本内（alpha）多样性指数和两个主要菌群（厚壁菌门和拟杆菌门；F/B 比值）与 BD 和 BMI 的关系。
在 69 名具有 BD 数据的女性中，39 名具有低 BD（BI-RADS I/II），30 名具有高 BD（BI-RADS III/IV）。BMI 与 BD 呈负相关（BD 较高的女性平均 BMI 分别为 23.8 和 28.0，p=1.07×10）。根据 Shannon（p=0.83）、Inverse Simpson（p=0.97）和 Chao1（p=0.31）指数，不同体重组 GM 多样性水平相似。F/B 比值和微生物多样性在 BD 较高的女性中高于 BD 较低的女性（p=0.35、0.14、0.15 和 0.17，分别为 F/B 比值、Shannon、Inverse Simpson 和 Chao1）。
需要在更大的研究中证实，BD 较高和较低的女性在 GM alpha 多样性和特定 GM 类群的流行率方面观察到的提示性差异。
