Department of Biological Sciences, Sunway University, Bandar Sunway, Malaysia.
Department of Clinical Sciences, College of Medicine, University of Sharjah, University City, Sharjah, United Arab Emirates.
Asian Pac J Cancer Prev. 2021 Mar 1;22(3):733-740. doi: 10.31557/APJCP.2021.22.3.733.
The overall aim was to determine whether gut bacteria of Columbia livia are a potential source of antitumour molecules.
Faecal and gut microbiota of Columbia livia were isolated, identified and conditioned media were prepared containing metabolites. Growth inhibition, lactate dehydrogenase cytotoxicity and cell survival assays were accomplished against cervical cancer cells. Next, liquid-chromatography mass spectrometry was conducted to elucidate the molecules present.
A plethora of bacteria from faecal matter and gastrointestinal tract were isolated. Selected conditioned media exhibited potent anticancer effects and displayed cytotoxicity to cervical cancer cells at IC50 concentration of 10.65 and 15.19 µg/ml. Moreover, cells treated with conditioned media exhibited morphological changes, including cell shrinking and rounding; indicative of apoptosis, when compared to untreated cells. A total of 111 and 71 molecules were revealed from these gut and faecal metabolites. The identity of 60 molecules were revealed including, dihydroxymelphalan. Nonetheless, 122 molecules remain unidentified and are the subject of future studies.
These findings suggest that gut bacteria of Columbia livia possess molecules, which may have anticancer activities. Further in silico testing and/or high throughput screening will determine potential anticancer properties of these molecules.
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本研究旨在确定哥伦比亚Liv 鸡的肠道细菌是否是抗肿瘤分子的潜在来源。
方法：分离、鉴定哥伦比亚 Liv 鸡的粪便和肠道微生物群，并制备含有代谢物的条件培养基。对宫颈癌细胞进行生长抑制、乳酸脱氢酶细胞毒性和细胞存活测定。然后，采用液相色谱-质谱联用技术阐明存在的分子。
结果：从粪便和胃肠道中分离出大量细菌。选择的条件培养基表现出很强的抗癌作用，对宫颈癌细胞的 IC50 浓度为 10.65 和 15.19 µg/ml 时表现出细胞毒性。此外，与未处理的细胞相比，用条件培养基处理的细胞表现出形态变化，包括细胞缩小和变圆，这表明发生了细胞凋亡。从这些肠道和粪便代谢物中总共揭示了 111 种和 71 种分子。鉴定出 60 种分子，包括二羟甲基苯丙氨酸。然而，仍有 122 种分子未被识别，这是未来研究的课题。
结论：这些发现表明哥伦比亚 Liv 鸡的肠道细菌具有可能具有抗癌活性的分子。进一步的计算机测试和/或高通量筛选将确定这些分子的潜在抗癌特性。
