Ningbo Eye Hospital, Ningbo, Zhejiang, 315040, China.
Department of Microbiology & Immunology, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China.
Talanta. 2021 Jun 1;228:122218. doi: 10.1016/j.talanta.2021.122218. Epub 2021 Feb 19.
pH plays an essential role in virtually all biological processes, which thus needs to be tightly regulated. Especially small changes in pH value in biological systems will affect the normal metabolic functions of animals and plants. Therefore, it is very important to accurately track changes in pH in biological systems. Herein, we rationally fabricated a new molecular pH probe (NBD-pbz) based on conjugation of the 2-Piperazin-1-yl-1,3-benzothiazole (pbz) and 7-nitro-1,2,3-benzoxadiazole (NBD) building blocks. The following test on NBD-pbz revealed that it can offer a sensitive and selective tracking of pH changes from 3.2 to 7.6 with pK 5.51, and not only in eukaryotic cells (especially the imaging of ocular tumor cell OCM-1) and zebrafish but also in mung bean sprouts via fluorometric turn on response and an intramolecular charge transfer (ICT) based working mechanism strategy. Therefore, this NBD-pbz probe not only replenishes the current repertory of molecular pH probes but also extends the application of pH probes to monitor pH variation in the plant kingdom, which will undoubtedly arouse greater research interest in the field.
pH 在几乎所有的生物过程中都起着至关重要的作用，因此需要严格控制。生物系统中 pH 值的微小变化会影响动植物的正常代谢功能。因此，准确跟踪生物系统中 pH 的变化非常重要。在此，我们基于 2-哌嗪-1-基-1,3-苯并噻唑（pbz）和 7-硝基-1,2,3-苯并恶二唑（NBD）构建块，合理设计了一种新型分子 pH 探针（NBD-pbz）。对 NBD-pbz 的测试表明，它可以在 pK 5.51 下从 3.2 到 7.6 进行灵敏和选择性的 pH 变化跟踪，不仅在真核细胞（特别是眼部肿瘤细胞 OCM-1 的成像）和斑马鱼中，而且在绿豆芽中，通过荧光开启响应和基于分子内电荷转移（ICT）的工作机制策略。因此，这种 NBD-pbz 探针不仅补充了当前的分子 pH 探针库，而且将 pH 探针的应用扩展到监测植物王国中的 pH 变化，这无疑会引起该领域更大的研究兴趣。
