Department of Polymer Engineering, Graduate School, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju 61186, Korea.
Drug Manufacturing Center, Daegu-Gyeongbuk Medical Innovation Foundation (DGMIF), Daegu 41061, Korea.
Int J Mol Sci. 2022 Apr 29;23(9):4949. doi: 10.3390/ijms23094949.
Recent interest in research on photoluminescent molecules due to their unique properties has played an important role in advancing the bioimaging field. In particular, small molecules and organic dots as probes have great potential for the achievement of bioimaging because of their desirable properties. In this review, we provide an introduction of probes consisting of fluorescent small molecules and polymers that emit light across the ultraviolet and near-infrared wavelength ranges, along with a brief summary of the most recent techniques for bioimaging. Since photoluminescence probes emitting light in different ranges have different goals and targets, their respective strategies also differ. Diverse and novel strategies using photoluminescence probes against targets have gradually been introduced in the related literature. Among recent papers (published within the last 5 years) on the topic, we here concentrate on the photophysical properties and strategies for the design of molecular probes, with key examples of in vivo photoluminescence research for practical applications. More in-depth studies on these probes will provide key insights into how to control the molecular structure and size/shape of organic probes for expanded bioimaging research and applications.
由于其独特的性质，近年来人们对光致发光分子的研究产生了浓厚的兴趣，这在推进生物成像领域方面发挥了重要作用。特别是小分子和有机点作为探针，由于其理想的性质，在实现生物成像方面具有巨大的潜力。在这篇综述中，我们介绍了由荧光小分子和聚合物组成的探针，它们在紫外线和近红外波长范围内发光，并简要总结了生物成像的最新技术。由于在不同波长范围内发射光的光致发光探针具有不同的目标和靶点，它们各自的策略也不同。针对靶点使用光致发光探针的各种新颖策略已逐渐在相关文献中得到介绍。在最近（过去 5 年内发表的）关于该主题的论文中，我们主要关注分子探针的光物理性质和设计策略，并以实际应用中体内光致发光研究的关键实例为例。对这些探针进行更深入的研究将为如何控制有机探针的分子结构和尺寸/形状提供重要的见解，从而扩展生物成像研究和应用。
