Gao Huan, Zhang Junfang, Wei Xuan, Zhu Qinshu, Wei Tianxiang
Jiangsu Collaborative Innovation Center of Biomedical Functional Materials and Jiangsu Key Laboratory of Biofunctional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing, 210023, People's Republic of China.
Jiangsu Collaborative Innovation Center of Biomedical Functional Materials and Jiangsu Key Laboratory of Biofunctional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing, 210023, People's Republic of China.
Talanta. 2021 Jun 1;228:122230. doi: 10.1016/j.talanta.2021.122230. Epub 2021 Feb 19.
Developing efficient and sensitive cytosensing method has great significance for the detection of low abundant circulating tumor cells (CTCs). Electrochemiluminescence (ECL) biosensor, as an attractive analytical tool, has shown a great potential in sensitive cell counting. Its detection efficiency is strongly dependent on the electrochemiluminescent materials, whose property is related to its morphology and surface vacancies. Herein, the ultrathin LuO-S nanosheets contain abundant oxygen vacancies were newly synthesized. Its special two-dimensional (2D) structure morphology and surface vacancy endowed it intensified and stable ECL emission. The possible mechanism was deduced from experiments and discussed. Then, through integrating with a DNA device cycle-amplification system plus signal conversion pretreatment, we constructed a crossed enhanced ECL cytosensing platform. In this system, the target cells were transformed into programmable sequences, which could be next coupled with DNA device cycle-amplification on the modified electrode surface. Using AgS quantum dots as the energy acceptor toward LuO-S donor, and CCRF-CEM cells (CEM) as the model CTCs, an enhanced ECL cytosensing platform was proposed, displaying good analytical performance for acute lymphoblastic leukemia cancer cell detection. The ECL signal responded proportionately on the CEM cells concentration in a wide range of 5 × 10 to 1 × 10 cells/mL, and a low detection limit of 10 cells/mL was obtained. This work provided an alternative way to design high-performance ECL luminophores, and also would be an effective solution for CTCs counting.
开发高效灵敏的细胞传感方法对于检测低丰度循环肿瘤细胞（CTC）具有重要意义。电化学发光（ECL）生物传感器作为一种有吸引力的分析工具，在灵敏的细胞计数方面显示出巨大潜力。其检测效率强烈依赖于电化学发光材料，而该材料的性质与其形态和表面空位有关。在此，新合成了含有丰富氧空位的超薄LuO-S纳米片。其特殊的二维（2D）结构形态和表面空位赋予了它增强且稳定的ECL发射。通过实验推导并讨论了可能的机制。然后，通过与DNA器件循环放大系统以及信号转换预处理相结合，构建了一个交叉增强的ECL细胞传感平台。在该系统中，目标细胞被转化为可编程序列，随后可与修饰电极表面的DNA器件循环放大相结合。以AgS量子点作为LuO-S供体的能量受体，并以CCRF-CEM细胞（CEM）作为模型CTC，提出了一种增强的ECL细胞传感平台，该平台在急性淋巴细胞白血病癌细胞检测中表现出良好的分析性能。ECL信号在5×10至1×10个细胞/mL的宽范围内与CEM细胞浓度成比例响应，并且获得了10个细胞/mL的低检测限。这项工作为设计高性能ECL发光体提供了一种替代方法，也将是CTC计数的有效解决方案。
