School of Pharmacy, Hebei Medical University, Shijiazhuang, 050017, China.
Hebei Key Laboratory of Forensic Medicine, Shijiazhuang, 050017, China.
Mikrochim Acta. 2022 Aug 6;189(9):323. doi: 10.1007/s00604-022-05428-w.
A new strategy is proposed for ultrasensitive detection of chlorogenic acid (CGA) by fabricating an electrochemiluminescence resonance energy transfer (ECL-RET) sensing platform. The novel system designed by introducing ruthenium-based 2D metal-organic framework nanosheets (Ru@Zn-MOF) as ECL acceptor and L-cysteine capped CdS quantum dots (L-CdS QDs) as ECL donor, exhibited good ECL response. The possible mechanism of the modified electrode surface reaction was discussed. Modifying of the electrode surface by application of L-CdS QDs directly on ultrathin MOF nanosheets greatly shortened the electron-transfer distance and reduce energy loss, therefore significantly improving the ECL efficiency. The prepared sensor demonstrated good stability and highly selective detection of the target molecule. Under optimal conditions, the constructed sensor for the detection of CGA exhibited a wide linear range from 1.0 × 10 to 1.0 × 10 mol·L and a low detection limit of 3.2 × 10 mol·L with a correction coefficient of 0.995. The recovery for spiked samples was calculated to be 94.4-109% and the RSD was 1.07-1.72% in real samples. The obtained sensor is considered to be a promising platform for CGA detection. Electrochemiluminescence resonance energy transfer (ECL-RET) sensing platform is used for the detection for chlorogenic acid.
提出了一种新的策略，通过构建电致化学发光共振能量转移（ECL-RET）传感平台来超灵敏检测绿原酸（CGA）。通过引入基于钌的二维金属有机骨架纳米片（Ru@Zn-MOF）作为 ECL 受体和 L-半胱氨酸封端的 CdS 量子点（L-CdS QDs）作为 ECL 供体，设计了新颖的系统，表现出良好的 ECL 响应。讨论了修饰电极表面反应的可能机制。通过将 L-CdS QDs 直接应用于超薄 MOF 纳米片上修饰电极表面，大大缩短了电子转移距离并减少了能量损失，从而显著提高了 ECL 效率。所制备的传感器表现出良好的稳定性和对目标分子的高选择性检测。在最佳条件下，构建的用于检测 CGA 的传感器在 1.0×10至 1.0×10 mol·L 的宽线性范围内表现出良好的性能，检测限低至 3.2×10 mol·L，校正系数为 0.995。在实际样品中，加标样品的回收率为 94.4-109%，RSD 为 1.07-1.72%。所得传感器被认为是 CGA 检测的有前途的平台。电致化学发光共振能量转移（ECL-RET）传感平台用于检测绿原酸。
