College of Horticulture, Northwest A and F University, Yangling, Shaanxi Province, 712100, China; State Key Laboratory of Crop Stress Biology in Arid Areas, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi Province, 712100, China.
Department of Genetics, Hazara University, Manshera, KPK, Pakistan.
Environ Res. 2021 Jun;197:111030. doi: 10.1016/j.envres.2021.111030. Epub 2021 Mar 24.
TAZ (transcriptional coactivator with PDZ-binding) zinc finger domains, also known as transcription adaptor putative zinc finger domains, that control diverse function in plant growth and development. Here, in the present study, we evaluated the role of the TAZ domain-containing gene in response to various heavy metals. Initially, we found a total of 3, 7, 8, 9, 9, 9, 7, 14, 6, 10, and 6 proteins containing TAZ domain in stiff brome, millet, sorghum, potato, pepper, maize, rice, apple, peach, pear, and tomato genome that could trigger the plant resistance against various heavy metals, respectively. Various in-silico approaches were applied such as duplication, phylogenetic analysis, and gene structure, to understand the basic features of the TAZ domain-containing genes in plants. Gene expression analyses were also performed under heavy metals (Cr, Zn, Ni, Cd, Co, Fe, Mn, and Pb). The results of quantitative real-time PCR analysis indicated that the TAZ gene family members were differentially expressed under different heavy metals. We further characterized the functions of the TAZ domain-containing gene under the heavy metal stresses by overexpressing the OsTAZ4 gene in Arabidopsis. The TAZ genes could promote plant resistance against various heavy metals by interacting with OsMYB34 and OsFHA9 transcription factors. The results will contribute to elucidate the relationship of TAZ proteins with heavy metals stresses and also ascertain the biological function in plant growth and development.
TAZ（与 PDZ 结合的转录共激活因子）锌指结构域，也称为转录接头假定锌指结构域，控制植物生长和发育的多种功能。在本研究中，我们评估了 TAZ 结构域包含基因在响应各种重金属中的作用。最初，我们在硬雀麦、谷子、高粱、土豆、辣椒、玉米、水稻、苹果、桃、梨和番茄基因组中发现了总共 3、7、8、9、9、9、7、14、6、10 和 6 种含有 TAZ 结构域的蛋白质，它们分别可以触发植物对各种重金属的抗性。应用了各种计算机方法，如重复、系统发育分析和基因结构，以了解植物中 TAZ 结构域包含基因的基本特征。还在重金属（Cr、Zn、Ni、Cd、Co、Fe、Mn 和 Pb）下进行了基因表达分析。定量实时 PCR 分析的结果表明，TAZ 基因家族成员在不同重金属下的表达存在差异。我们通过在拟南芥中过表达 OsTAZ4 基因进一步表征了 TAZ 结构域包含基因在重金属胁迫下的功能。TAZ 基因可以通过与 OsMYB34 和 OsFHA9 转录因子相互作用来促进植物对各种重金属的抗性。这些结果将有助于阐明 TAZ 蛋白与重金属胁迫的关系，并确定其在植物生长和发育中的生物学功能。
