改良的无需匀浆的氢氧化钠消化法用于从肾组织中提取庆大霉素。
Improved sodium hydroxide digestion method without homogenization for extraction of gentamicin from renal tissue.
作者信息
Brown S A, Sugimoto K, Smith G G, Garry F B
机构信息
Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine, Texas A & M University, College Station 77843.
出版信息
Antimicrob Agents Chemother. 1988 Apr;32(4):595-7. doi: 10.1128/AAC.32.4.595.
Abstract
Recovery of gentamicin from renal cortical tissue (assayed by fluorescence polarization immunoassay) was highest (P less than 0.05) when using a sodium hydroxide (NaOH) digestion procedure without homogenization (90.0 +/- 5.4%), followed by homogenization with NaOH digestion (85.8 +/- 7.7%) and homogenization with trichloroacetic acid precipitation (84.4 +/- 3.3%). Simple homogenization recovered the least gentamicin (59.0 +/- 5.2%; P less than 0.05).
摘要
通过荧光偏振免疫分析法测定，在不进行匀浆的情况下使用氢氧化钠（NaOH）消化程序时，从肾皮质组织中回收庆大霉素的效率最高（P<0.05）（90.0±5.4%），其次是NaOH消化后匀浆（85.8±7.7%）和三氯乙酸沉淀后匀浆（84.4±3.3%）。单纯匀浆回收的庆大霉素最少（59.0±5.2%；P<0.05）。
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