Graduate School of Environmental Science, Hokkaido University, Sapporo, Japan.
Faculty of Science, Hokkaido University, Sapporo, Japan.
Dev Genes Evol. 2021 Jul;231(3-4):85-93. doi: 10.1007/s00427-021-00674-z. Epub 2021 Mar 27.
A polka-dotted fruit fly, Drosophila guttifera, has a unique pigmentation pattern on its wings and is used as a model for evo-devo studies exploring the mechanism of evolutionary gain of novel traits. In this species, a morphogen-encoding gene, wingless, is expressed in species-specific positions and induces a unique pigmentation pattern. To produce some of the pigmentation spots on wing veins, wingless is thought to be expressed in developing campaniform sensillum cells, but it was unknown which of the four cell types there express(es) wingless. Here we show that two of the cell types, dome cells and socket cells, express wingless, as indicated by in situ hybridization together with immunohistochemistry. This is a unique case in which non-neuronal SOP (sensory organ precursor) progeny cells produce Wingless as an inducer of pigmentation pattern formation. Our finding opens a path to clarifying the mechanism of evolutionary gain of a unique wingless expression pattern by analyzing gene regulation in dome cells and socket cells.
一只具有斑点的果蝇，果蝇 guttifera，其翅膀上具有独特的色素图案，被用作探索进化获得新特征的机制的演化发育研究的模型。在这个物种中，一种形态发生素编码基因，无翅，在特定的物种位置表达，并诱导独特的色素图案。为了在翅脉上产生一些色素斑点，无翅被认为在发育中的杯状感觉细胞中表达，但尚不清楚四个细胞类型中的哪一个表达无翅。在这里，我们通过原位杂交结合免疫组织化学表明，两种细胞类型，穹顶细胞和插座细胞，表达无翅。这是一个独特的案例，其中非神经元 SOP（感觉器官前体）祖细胞产生 Wingless 作为色素图案形成的诱导物。我们的发现为通过分析穹顶细胞和插座细胞中的基因调控来阐明独特的无翅表达模式的进化获得机制开辟了道路。
