Department of Veterinary Internal Medicine, College of Veterinary Medicine, Konkuk University, Seoul 05029, Korea.
J Vet Sci. 2021 Mar;22(2):e17. doi: 10.4142/jvs.2021.22.e17.
spp. is an important conditional pathogen in humans and animals. However, due to the indiscriminate use of antibiotics, the incidence of antimicrobial resistance has increased.
The purpose of this study was to investigate antimicrobial resistance in strains of strains and the phylogenetic relatedness of extended-spectrum cephalosporin (ESC)-resistance among strains isolated from clinically ill companion animals.
A total of 336 clinical specimens were collected from animal hospitals. Identification of species, determination of minimum inhibitory concentrations, detection of ESC resistance genes, polymerase chain reaction-based replicon typing of plasmids by conjugation, and multilocus sequence typing were performed.
Forty-three strains were isolated and, subsequently, 28 were identified as , 11 as , and 4 as . Eleven strains were isolated from feces, followed by 10 from ear, 7 from the nasal cavity, 6 from urine, 5 from genitals, and 4 from skin. isolates showed more than 40% resistance to penicillin, cephalosporin, fluoroquinolone, and aminoglycoside. ESCresistance genes, CTX-M groups (CTX-M-3, CTX-M-15, and CTX-M-65), and AmpC (CMY-2 and DHA-1) were most common in the strains. Some carrying CTX-M or AmpC were transferred via IncFII plasmids. Two sequence types, ST709 and ST307, from were most common.
In conclusion, this is the first report on the prevalence, ESCresistance genotypes, and sequence types of strains isolated from clinically ill companion animals. The combination of infectious diseases and antimicrobial resistance by in companion animals suggest that, in clinical veterinary, antibiotic selection should be made carefully and in conjunction with the disease diagnosis.
spp. 是人类和动物的重要条件致病菌。然而，由于抗生素的滥用，抗菌药物耐药性的发生率有所增加。
本研究旨在调查从患病伴侣动物中分离的 菌株的抗菌药物耐药性以及对扩展谱头孢菌素（ESC）耐药的菌株之间的系统发育关系。
从动物医院采集了 336 份临床标本。对 种进行鉴定，测定最小抑菌浓度，检测 ESC 耐药基因，通过接合进行基于聚合酶链反应的质粒复制子分型，以及多位点序列分型。
共分离出 43 株 株，其中 28 株鉴定为 ，11 株鉴定为 ，4 株鉴定为 。11 株分离自粪便，其次是 10 株分离自耳朵，7 株分离自鼻腔，6 株分离自尿液，5 株分离自生殖器，4 株分离自皮肤。 分离株对青霉素、头孢菌素、氟喹诺酮类和氨基糖苷类的耐药率超过 40%。在 菌株中最常见的 ESC 耐药基因、CTX-M 组（CTX-M-3、CTX-M-15 和 CTX-M-65）和 AmpC（CMY-2 和 DHA-1）。一些携带 CTX-M 或 AmpC 的 菌株通过 IncFII 质粒转移。最常见的 分离株的序列类型为 ST709 和 ST307。
总之，这是首次报道从患病伴侣动物中分离的 菌株的流行率、ESC 耐药基因型和序列类型。伴侣动物中 引起的传染病和抗菌药物耐药性的结合表明，在临床兽医中，应谨慎选择抗生素，并结合疾病诊断。
