Sebola Dikeledi C, Oguttu James W, Kock Marleen M, Qekwana Daniel N
Section Veterinary Public Health, Department of Paraclinical Sciences, Faculty of Veterinary Science, University of Pretoria, Pretoria, South Africa.
Department of Agriculture and Animal Health, College of Agriculture and Environmental Sciences, University of South Africa, Johannesburg, South Africa.
Vet World. 2023 Sep;16(9):1880-1888. doi: 10.14202/vetworld.2023.1880-1888. Epub 2023 Sep 17.
and are opportunistic bacterial pathogens responsible for hospital-acquired infections in veterinary medicine. Infection with these bacteria always requires urgent antimicrobial therapy. However, there is no evidence of studies that have investigated the antimicrobial drug resistance profile of these organisms in a veterinary setting in South Africa. This study investigated the antimicrobial resistance (AMR) patterns of and from clinical specimens obtained from dogs presented at a veterinary academic hospital. The findings of this study contribute to an improved understanding of the AMR profile of these bacteria in veterinary medicine.
Retrospective data of clinical samples from dogs that were positive for and between 2007 and 2013 were used in this study. The antimicrobial susceptibility of the isolates was determined using the disk diffusion method following the Clinical and Laboratory Standards Institute guidelines. The isolates were subjected to a panel of 20 antibiotics, while isolates were subjected to a panel of 22 antibiotics. Data were analyzed using descriptive statistics and presented using tables and figures.
Twenty (n = 20) isolates were isolated from bronchoalveolar lavage, foreign objects, bone, urine, skin, blood, ear, nasal, and oral cavity. Almost all (95%, 19/20) isolates were resistant to at least one antibiotic, and 60% (12/20) were multidrug-resistant (MDR). (n = 56) was isolated from urine, foreign objects, abscesses, ears, eyes, tracheal aspirations, bronchoalveolar lavages, eyes, abdominal aspirates, anal glands, bones, and intestinal and lung biopsies. All (100%, 56/56) isolates were resistant to at least one antibiotic, and 98% (55/56) were MDR.
Both and were isolated in various clinical tissue samples and exhibited a high prevalence of resistance to multiple antibiotics. In addition, these bacteria exhibited a high prevalence of resistance to β-lactam compared to other classes of antibiotics, which is likely to impact treatment options and patient prognosis.
[细菌名称1]和[细菌名称2]是兽医学中导致医院获得性感染的机会性病原菌。感染这些细菌总是需要紧急抗菌治疗。然而，没有证据表明有研究在南非的兽医环境中调查过这些微生物的抗菌药物耐药性情况。本研究调查了从一家兽医学术医院就诊的犬只的临床标本中分离出的[细菌名称1]和[细菌名称2]的抗菌耐药性（AMR）模式。本研究的结果有助于更好地了解这些细菌在兽医学中的AMR情况。
本研究使用了2007年至2013年间[细菌名称1]和[细菌名称2]检测呈阳性的犬只临床样本的回顾性数据。按照临床和实验室标准协会的指南，使用纸片扩散法测定分离株的抗菌敏感性。[细菌名称1]分离株接受一组20种抗生素检测，而[细菌名称2]分离株接受一组22种抗生素检测。使用描述性统计方法分析数据，并以表格和图表形式呈现。
从支气管肺泡灌洗、异物、骨骼、尿液、皮肤、血液、耳朵、鼻腔和口腔中分离出20株[细菌名称1]分离株。几乎所有（95%，19/20）的[细菌名称1]分离株对至少一种抗生素耐药，60%（12/20）为多重耐药（MDR）。从尿液、异物、脓肿、耳朵、眼睛、气管吸出物、支气管肺泡灌洗、眼睛、腹腔吸出物、肛门腺、骨骼以及肠道和肺部活检中分离出56株[细菌名称2]。所有（100%，56/56）的[细菌名称2]分离株对至少一种抗生素耐药，98%（55/56）为MDR。
[细菌名称1]和[细菌名称2]均在各种临床组织样本中被分离出来，并且对多种抗生素呈现出高耐药率。此外，与其他类别的抗生素相比，这些细菌对β-内酰胺类抗生素的耐药率较高，这可能会影响治疗选择和患者预后。
