College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, Shandong, 271000, China; State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, Shandong, 271000, China; Shandong Collaborative Innovation Center for Fruit & Vegetable Production With High Quality and Efficiency, Tai'an, Shandong, 271000, China.
Shandong Academy of Agricultural Sciences, Jinan, Shandong, 250100, China.
Plant Sci. 2021 May;306:110874. doi: 10.1016/j.plantsci.2021.110874. Epub 2021 Mar 6.
EARLY BUD-BREAK 1 (EBB1) can promote bud break, and this function is likely conserved in woody plants. To get a more comprehensive understand of its function, peach (Prunus persica var. nectarina cultivar Zhongyou 4) PpEBB1 was overexpressed in Arabidopsis; the resultant phenotypes, including curved leaves, abnormal development of floral organs and low seed set, were similar to those of DORNRÖSCHEN-LIKE (DRNL) overexpression, indicating that PpEBB1 was a putative ortholog of AtDRNL. PpEBB1 bound to the GCC box-like element in the STYLISH1/SHI RELATED SEQUENCE5 (STY1/SRS5) promoter of peach, which has been proposed to occur in Arabidopsis as well. A GCC box-like element was also found in the YUCCA1 (YUC1) promoter, and PpEBB1 could bind to this element and activate the expression of YUC1. In addition to the elevated auxin content in the PpEBB1-oe plants as observed in our previous study, these results suggest that PpEBB1 can regulate auxin biosynthesis by directly activating related genes. Besides, we screened a zinc finger RING-finger protein, MYB30-INTERACTING E3 LIGASE 1 (PpMIEL1), showing interaction with PpEBB1, suggesting that the stability of PpEBB1 might be influenced by PpMIEL1 through ubiquitination.
早期芽破裂 1（EBB1）可以促进芽破裂，并且这个功能在木本植物中可能是保守的。为了更全面地了解其功能，在拟南芥中过表达了桃（Prunus persica var. nectarina 栽培品种中油 4）PpEBB1；其表型包括弯曲的叶片、花器官发育异常和结实率低，与 DORNRÖSCHEN-LIKE（DRNL）过表达的表型相似，表明 PpEBB1 是 AtDRNL 的假定同源物。PpEBB1 与桃的 STYLISH1/SHI RELATED SEQUENCE5（STY1/SRS5）启动子中的 GCC 盒样元件结合，据推测该元件也存在于拟南芥中。GCC 盒样元件也存在于 YUCCA1（YUC1）启动子中，PpEBB1 可以与该元件结合并激活 YUC1 的表达。除了我们之前的研究中观察到 PpEBB1-oe 植物中生长素含量升高外，这些结果表明 PpEBB1 可以通过直接激活相关基因来调节生长素的生物合成。此外，我们筛选了一个锌指 RING 指蛋白 MYB30-INTERACTING E3 LIGASE 1（PpMIEL1），其与 PpEBB1 相互作用，表明 PpEBB1 的稳定性可能通过泛素化受到 PpMIEL1 的影响。
