Zhao Xuehui, Wen Binbin, Li Chen, Tan Qiuping, Liu Li, Chen Xiude, Li Ling, Fu Xiling
College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, China.
State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, China.
Front Plant Sci. 2021 Jun 17;12:681283. doi: 10.3389/fpls.2021.681283. eCollection 2021.
Shoot branching is an important adaptive trait that determines plant architecture. In a previous study, the () gene in peach ( var. ) cultivar Zhongyou 4 was transformed into poplar (). poplar showed a more branched phenotype. To understand the potential mechanisms underlying the EBB1-mediated branching, transcriptomic and proteomics analyses were used. The results showed that a large number of differentially expressed genes (DEGs)/differentially expressed proteins (DEPs) associated with light response, sugars, brassinosteroids (BR), and nitrogen metabolism were significantly enriched in poplar. In addition, contents of sugars, BR, and amino acids were measured. Results showed that PpEBB1 significantly promoted the accumulation of fructose, glucose, sucrose, trehalose, and starch. Contents of brassinolide (BL), castasterone (CS), and 6-deoxocathasterone (6-deoxoCS) were all significantly changed with overexpressing . Various types of amino acids were measured and four of them were significantly improved in poplar, including aspartic acid (Asp), arginine (Arg), cysteine (Cys), and tryptohpan (Trp). Taken together, shoot branching is a process controlled by a complex regulatory network, and PpEBB1 may play important roles in this process through the coordinating multiple metabolic pathways involved in shoot branching, including light response, phytohormones, sugars, and nitrogen.
枝条分枝是决定植物形态的重要适应性性状。在先前的一项研究中，桃（品种中油4）的（）基因被转入杨树（）。转基因杨树表现出更多分枝的表型。为了了解EBB1介导的分枝潜在机制，采用了转录组学和蛋白质组学分析。结果表明，与光反应、糖类、油菜素甾醇（BR）和氮代谢相关的大量差异表达基因（DEGs）/差异表达蛋白（DEPs）在转基因杨树中显著富集。此外，还测定了糖类、BR和氨基酸的含量。结果表明，PpEBB1显著促进了果糖、葡萄糖、蔗糖、海藻糖和淀粉的积累。过表达时，油菜素内酯（BL）、菜甾酮（CS）和6-脱氧菜甾酮（6-deoxoCS）的含量均发生了显著变化。测定了各种氨基酸，其中4种在转基因杨树中显著增加，包括天冬氨酸（Asp）、精氨酸（Arg）、半胱氨酸（Cys）和色氨酸（Trp）。综上所述，枝条分枝是一个由复杂调控网络控制的过程，PpEBB1可能通过协调参与枝条分枝的多种代谢途径，包括光反应、植物激素、糖类和氮，在这一过程中发挥重要作用。
