Fazaeli Asghar, Kohansal Mohammad Hasan, Spotin Adel, Haniloo Ali, Nourian Abbasali, Khiabani Alireza, Siyadatpanah Abolghasem, Norouzi Roghayeh, Nissapatorn Veeranoot
Department of Parasitology and Mycology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
School of Medicine, Bam University of Medical Sciences, Bam, Iran.
Vet World. 2021 Feb;14(2):419-425. doi: 10.14202/vetworld.2021.419-425. Epub 2021 Feb 16.
is one of the most common enteric protozoan parasites in vertebrates, such as humans, domestic and wild animals, causing giardiasis. To the best of our knowledge, little is known about the genetic diversity of assemblages. This study aimed to identify genetic diversity of assemblages in Iranian stray dogs.
A total of 450 fecal samples were collected from 2015 to 2016 from stray dogs of Northwest Iran. All specimens were observed microscopically following concentration and flotation techniques. Subsequently, DNA samples were extracted, amplified, and sequenced targeting the gene.
The overall prevalence of in infected dogs was estimated at 1.6%, based on microscopic and molecular diagnoses. Sequencing and phylogenetic analyses indicated a high level of genetic diversity of assemblage C (haplotype diversity; 0.802).
The pairwise sequence distances between the identified isolates of assemblage C showed an intradiversity of 0.3%-1.3% and identity of 98.7%-100%. Current findings indicate that a significant genetic diversity of assemblage C haplotypes is unequivocally circulates among stray dogs in Northwest Iran.
[寄生虫名称]是脊椎动物（如人类、家畜和野生动物）中最常见的肠道原生动物寄生虫之一，可引起贾第虫病。据我们所知，关于[寄生虫名称]不同组合的遗传多样性了解甚少。本研究旨在鉴定伊朗流浪狗中[寄生虫名称]不同组合的遗传多样性。
2015年至2016年期间，从伊朗西北部的流浪狗身上共采集了450份粪便样本。所有标本经过浓缩和浮选技术处理后进行显微镜观察。随后，提取DNA样本，针对[目标基因名称]基因进行扩增和测序。
基于显微镜和分子诊断，感染狗中[寄生虫名称]的总体患病率估计为1.6%。测序和系统发育分析表明组合C具有高度的遗传多样性（单倍型多样性；0.802）。
鉴定出的组合C分离株之间的成对序列距离显示出0.3%-1.3%的种内多样性和98.7%-100%的同一性。目前的研究结果表明，组合C单倍型的显著遗传多样性在伊朗西北部的流浪狗中明确存在。
