Soliman Essam S, Hamad Rania T, Abdallah Mona S
Department of Animal Hygiene, Zoonosis, and Animal Behavior, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt.
Department of Pathology, Faculty of Veterinary Medicine, Menoufia University, Al Minufya 33511, Egypt.
Vet World. 2021 Feb;14(2):523-536. doi: 10.14202/vetworld.2021.523-536. Epub 2021 Feb 26.
Probiotics improve intestinal balance through bacterial antagonism and competitive exclusion. This study aimed to investigate the o antimicrobial activity, as well as the o preventive, immunological, productive, and histopathological modifications produced by probiotic s.
The antimicrobial activities of (5×10 CFU/g; 0.5, 1.0*, 1.5, and 2.0 g/L) were tested against O157: H7, Typhimurium, , and after exposure times of 0.25, 0.5, 1, and 2 h using minimal inhibitory concentration procedures. A total of 320 1-day-old female Ross broiler chickens were divided into five groups. Four out of the five groups were supplemented with 0.5, 1.0*, 1.5, and 2.0 g/L probiotic from the age of 1 day old. Supplemented 14-day-old broiler chickens were challenged with only O157: H7 (4.5×10 CFU/mL) and . Typhimurium (1.2×10 CFU/mL). A total of 2461 samples (256 microbial-probiotic mixtures, 315 sera, 315 duodenal swabs, and 1575 organs) were collected.
The results revealed highly significant (p<0.001) killing rates at all-time points in 2.0 g/L : 99.9%, 90.0%, 95.6%, and 98.8% against , . Typhimurium, , and , respectively. Broilers supplemented with 1.5 and 2.0 g/L revealed highly significant increases (p<0.01) in body weights, weight gains, carcass weights, edible organs' weights, immune organs' weights, biochemical profile, and immunoglobulin concentrations, as well as highly significant declines (p<0.01) in total bacterial, , and counts. Histopathological photomicrographs revealed pronounced improvements and near-normal pictures of the livers and hearts of broilers with lymphoid hyperplasia in the bursa of Fabricius, thymus, and spleen after supplementation with 2.0 g/L .
The studies revealed that 1.5-2.0 g of probiotic at a concentration of 5×10 CFU/g/L water was able to improve performance, enhance immunity, and tissue architecture, and produce direct antimicrobial actions.
益生菌通过细菌拮抗和竞争排斥作用改善肠道平衡。本研究旨在探究益生菌产生的抗菌活性以及预防、免疫、生产性能和组织病理学方面的改变。
采用最低抑菌浓度法，在暴露时间为0.25、0.5、1和2小时后，测试（5×10 CFU/g；0.5、1.0*、1.5和2.0 g/L）对O157:H7、鼠伤寒沙门氏菌、[此处原文有缺失]和[此处原文有缺失]的抗菌活性。总共320只1日龄雌性罗斯肉鸡被分为五组。五组中的四组从1日龄开始分别添加0.5、1.0*、1.5和2.0 g/L的益生菌[此处原文有缺失]。对14日龄添加益生菌的肉鸡仅用O157:H7（4.5×10 CFU/mL）和鼠伤寒沙门氏菌（1.2×10 CFU/mL）进行攻毒。共采集了2461个样本（256个微生物 - 益生菌混合物、315份血清、315份十二指肠拭子和1575个器官）。
结果显示，2.0 g/L[此处原文有缺失]在所有时间点的杀灭率均极显著（p<0.001）：对[此处原文有缺失]、鼠伤寒沙门氏菌、[此处原文有缺失]和[此处原文有缺失]的杀灭率分别为99.9%、90.0%、95.6%和98.8%。添加1.5和2.0 g/L[此处原文有缺失]的肉鸡在体重、增重、胴体重、可食器官重量、免疫器官重量、生化指标和免疫球蛋白浓度方面均有极显著增加（p<0.01），同时总细菌数、[此处原文有缺失]和[此处原文有缺失]计数极显著下降（p<0.01）。组织病理学显微照片显示，添加2.0 g/L[此处原文有缺失]后，法氏囊、胸腺和脾脏出现淋巴样增生，肉鸡的肝脏和心脏有明显改善且接近正常状态。
研究表明，浓度为5×10 CFU/g/L水的1.5 - 2.0 g益生菌[此处原文有缺失]能够改善生产性能、增强免疫力和组织结构，并产生直接抗菌作用。
