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鉴定和表征EvpQ，一种编码在移动遗传元件上的新型VI型分泌系统效应蛋白。 （注：原文最后“in.”后面内容缺失，以上翻译根据完整意思推测补充了部分内容使句子完整通顺）

Identification and Characterization of EvpQ, a Novel T6SS Effector Encoded on a Mobile Genetic Element in .

作者信息

Li Duan You, Liu Ying Li, Liao Xiao Jian, He Tian Tian, Sun Shan Shan, Nie Pin, Xie Hai Xia

机构信息

State Key Laboratory of Freshwater Ecology and Biotechnology, Key Laboratory of Aquaculture Disease Control, Ministry of Agriculture, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China.

College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, China.

出版信息

Front Microbiol. 2021 Mar 11;12:643498. doi: 10.3389/fmicb.2021.643498. eCollection 2021.

DOI:10.3389/fmicb.2021.643498
PMID:33776977
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7991086/
Abstract

In this study, a hypothetical protein (ORF02740) secreted by was identified. We renamed the ORF02740 protein as EvpQ, which is encoded by a mobile genetic element (MGE) in genome. The gene is spaced by 513 genes from type VI secretion system (T6SS) gene cluster. Low GC content, three tRNA, and three transposase genes nearby define this MGE that localizes as a genomic island. Sequence analysis reveals that EvpQ shares a conserved domain of C70 family cysteine protease and shares 23.91% identity with T3SS effector AvrRpt2 of phytopathogenic Instead, EvpQ of is proved to be secreted at a T6SS-dependent manner, and it can be translocated into host cells. EvpQ is thereof a novel T6SS effector. Significantly decreased competitive index of Δ strain in blue gourami fish (0.53 ± 0.27 in head kidney and 0.44 ± 0.19 in spleen) indicates that EvpQ contributes to the pathogenesis of . At 8-, 18-, and 24-h post-subculture into DMEM, the transcription of was found to be negatively regulated by Fur and positively regulated by EsrC, and the steady-state protein levels of EvpQ are negatively controlled by RpoS. Our study lays a foundation for further understanding the pathogenic role of T6SS in edwardsiellosis.

摘要

在本研究中，鉴定出了由[具体细菌名称未给出]分泌的一种假定蛋白（ORF02740）。我们将ORF02740蛋白重命名为EvpQ，它由[具体细菌名称未给出]基因组中的一个可移动遗传元件（MGE）编码。[具体细菌名称未给出]基因与VI型分泌系统（T6SS）基因簇间隔513个基因。[具体细菌名称未给出]附近的低GC含量、三个tRNA和三个转座酶基因定义了这个定位为基因组岛的MGE。序列分析表明，EvpQ与C70家族半胱氨酸蛋白酶共享一个保守结构域，与植物致病[具体细菌名称未给出]的III型分泌系统效应子AvrRpt2具有23.91%的同一性。相反，[具体细菌名称未给出]的EvpQ被证明以T6SS依赖的方式分泌，并且它可以转运到宿主细胞中。因此，EvpQ是一种新型的T6SS效应子。蓝曼龙鱼中Δ[具体细菌名称未给出]菌株的竞争指数显著降低（头肾中为0.53±0.27，脾脏中为0.44±0.19），表明EvpQ有助于[具体细菌名称未给出]的致病作用。在接种到DMEM中8小时、18小时和24小时后，发现[具体细菌名称未给出]的转录受到Fur的负调控和EsrC的正调控，并且EvpQ的稳态蛋白水平受到RpoS的负调控。我们为进一步了解T6SS在爱德华氏菌病中的致病作用奠定了基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47f9/7991086/7ec5991eea7e/fmicb-12-643498-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47f9/7991086/d9e2043fb0fe/fmicb-12-643498-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47f9/7991086/b139c85fbb4d/fmicb-12-643498-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47f9/7991086/8590f00ee8ed/fmicb-12-643498-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47f9/7991086/1ecf01189d81/fmicb-12-643498-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47f9/7991086/c18489bea75c/fmicb-12-643498-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47f9/7991086/7ec5991eea7e/fmicb-12-643498-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47f9/7991086/d9e2043fb0fe/fmicb-12-643498-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47f9/7991086/b139c85fbb4d/fmicb-12-643498-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47f9/7991086/8590f00ee8ed/fmicb-12-643498-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47f9/7991086/1ecf01189d81/fmicb-12-643498-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47f9/7991086/c18489bea75c/fmicb-12-643498-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47f9/7991086/7ec5991eea7e/fmicb-12-643498-g006.jpg

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