Wei De, Shen Shanghang, Lin Kun, Lu Feng, Zheng Pengfeng, Wu Shizhong, Kang Dezhi
Department of Neurosurgery, Fujian Provincial Hospital South Branch, Fuzhou, China.
Department of Neurosurgery, Shengli Clinical Medical College of Fujian Medical University, Fuzhou, China.
Front Genet. 2021 Mar 11;12:611442. doi: 10.3389/fgene.2021.611442. eCollection 2021.
Glioblastoma (GBM) is one of the most common and fatal malignancies worldwide, while its prognostic biomarkers are still being explored. This study aims to identify potential genes with clinical and prognostic significance by integrating bioinformatics analysis and investigating their function in HNSCC. Based on the Single-cell RNA sequencing (scRNA-seq) results of H3K27M-glioma cells, computational bioinformatics methods were employed for selecting prognostic biomarker for GBM. The protein NPC2 (NPC Intracellular Cholesterol Transporter 2), which has been shown to be related to lipoprotein metabolism and innate immune system, was identified to be upregulated in GBM. NPC2 showed a relatively higher expression in GBM samples, and a negative correlation with tumor purity and tumor infiltrating immune cells. Additionally, NPC2 was knocked down in U87-MG and U251 cells line, and cell proliferation and migration capability were evaluated with CCK-8, scratch and transwell assay, respectively. Cytological experiments has shown that NPC2 overexpression inhibited GBM cells proliferation and migration, indicating its important role in GBM progression. This is the first investigation into the prognostic value of NPC2 interact with GBM. The potential molecular factor NPC2 have been identified as a prognostic biomarker for GBM.
胶质母细胞瘤（GBM）是全球最常见且致命的恶性肿瘤之一，其预后生物标志物仍在探索中。本研究旨在通过整合生物信息学分析并研究其在头颈部鳞状细胞癌（HNSCC）中的功能，来鉴定具有临床和预后意义的潜在基因。基于H3K27M - 胶质瘤细胞的单细胞RNA测序（scRNA - seq）结果，采用计算生物信息学方法为GBM选择预后生物标志物。已证明与脂蛋白代谢和先天免疫系统相关的蛋白质NPC2（NPC细胞内胆固醇转运蛋白2）在GBM中上调。NPC2在GBM样本中表达相对较高，且与肿瘤纯度和肿瘤浸润免疫细胞呈负相关。此外，在U87 - MG和U251细胞系中敲低NPC2，并分别用CCK - 8、划痕和Transwell实验评估细胞增殖和迁移能力。细胞学实验表明，NPC2过表达抑制GBM细胞增殖和迁移，表明其在GBM进展中起重要作用。这是首次对NPC2与GBM相互作用的预后价值进行研究。潜在分子因子NPC2已被鉴定为GBM的预后生物标志物。
